Effect of inhibition of cyclooxygenase on pre- and postjunctional actions of peroxides in the iris-ciliary body.
Effect of inhibition of cyclooxygenase on pre- and postjunctional actions of peroxides in the iris-ciliary body.
复制标题
环加氧酶的抑制对虹膜睫状体中过氧化物的前和后作用的影响。
DOI：
10.1023/a:1007568227387
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发表时间：
2000
影响因子：
4.4
通讯作者：
Ohia,SE
中科院分区：
文献类型：
--
作者：
Graham,J;Awe,SO;LeDay,AM;Rice,JC;Ramos,G;Harris,LC;Opere,CA;Ohia,SE
在本研究中，我们研究了氟比洛芬（FBF）抑制环氧合酶（考克斯）对过氧化物诱导的场刺激牛虹膜释放[3 H]去甲肾上腺素（[3 H]NE）的增强作用。此外，FBF的效果进行了检查，对过氧化氢引起的衰减引起的收缩卡巴胆碱对这个组织。制备虹膜用于神经递质释放的研究和用于测量离体收缩张力。FBF（10 μM）预处理组织使H2 O2浓度-反应曲线显著（P < 0.001）下移，并减弱过氧化氢枯烯（CuOOH）引起的[3 H]NE释放的增强作用。FBF（10 μM）可部分阻止H2 O2（300 μM）和CuOOH（300 μM）引起的卡巴胆碱收缩的衰减。我们的结论是，前列腺素类化合物的生物合成的抑制减少了两个prejunctional刺激作用的H2 O2和CuOOH对交感神经传递和抑制作用的过氧化物对卡巴胆碱诱导的收缩在牛离体虹膜。
In the present study, we investigated the effect of inhibition of cyclooxygenase (COX) with flurbiprofen (FBF) on peroxide-induced enhancement of field-stimulated [3H]norepinephrine ([3H]NE) release from bovine isolated irides. Furthermore, the effect of FBF was examined on peroxide-induced attenuation of contractions evoked by carbachol on this tissue. Irides were prepared for studies of neurotransmitter release and for measurement of contractile tension in vitro. Pretreatment of tissues with FBF (10 μM) caused significant (P < 0.001) rightward shifts of concentration-response curves to H2O2and also decreased cumene hydroperoxide (cuOOH)-induced enhancement of evoked [3H]NE release. FBF (10 μM) partially prevented the attenuation of carbachol-induced contractions induced by H2O2(300 μM) and cuOOH (300 μM). We conclude that inhibition of the biosynthesis of prostanoids reduced both the prejunctional stimulatory effects of H2O2and cuOOH on sympathetic neurotransmission and inhibitory effects of peroxides on carbachol-induced contractions the in the bovine isolated iris.