Automated ordering of fingerprinted clones
Automated ordering of fingerprinted clones
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DOI：
10.1093/bioinformatics/bth079
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发表时间：
2004-05-22
期刊：
影响因子：
5.8
通讯作者：
Marra, MA
中科院分区：
文献类型：
--
作者：
Flibotte, S;Chiu, R;Marra, MA
动机：目前需要大量的人为干预，以产生高质量的指纹为基础的物理地图的genomic study.Results：一种算法已经开发和实施，以自动排序重叠群内的指纹克隆。由此产生的软件，名为珊瑚（克隆订购AL出租），已在地图上进行了测试，以前已手动编辑和来自计算机模拟的地图。人类基因组的指纹图和DNA序列为CORAL提供了另一个测试。测量结果表明，CORAL的表现明显优于目前大多数实验室使用的软件，以远远超过手工操作的吞吐量订购指纹克隆。S.
Motivation: A considerable amount of human intervention is currently required to produce high-quality fingerprint-based physical maps for genomic studies.Results: An algorithm has been developed and implemented to automatically order fingerprinted clones within contigs. The resulting software, named CORAL (Clone ORdering ALgorithm), has been tested on maps that have previously been manually edited and on maps derived from in silico simulations. The fingerprint map and DNA sequence of the human genome has provided an additional test to CORAL. Measurements suggest that CORAL performs significantly better than the software currently used by most laboratories to order fingerprinted clones at throughputs far exceeding those that can be achieved manually. s.