Transport and metabolism of glycerophosphodiesters produced through phospholipid deacylation.
Transport and metabolism of glycerophosphodiesters produced through phospholipid deacylation.
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DOI：
10.1016/j.bbalip.2006.04.013
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发表时间：
2007-03
期刊：
影响因子：
--
通讯作者：
J. Patton-Vogt
中科院分区：
文献类型：
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作者：
J. Patton-Vogt
磷脂脱酰化导致甘油磷酸二酯和游离脂肪酸的形成。在酿酒酵母中，已经表征了具有磷脂酶B（脱酰）活性的四种基因产物（PLB 1、PLB 2、PLB 3、NTE 1），并且这些活性占迄今观察到的甘油磷酸二酯产生的大部分（如果不是全部的话）。甘油磷酸二酯本身被甘油磷酸二酯磷酸二酯酶水解成甘油-3-磷酸和相应的醇。虽然在S.当然，还有其他的存在。内部和外部甘油磷酸二酯（主要是甘油磷酸胆碱和甘油磷酸肌醇）是由于S.啤酒。由GIT 1基因编码的通透酶通过质膜输入细胞外甘油磷酸二酯，其水解产物可以为细胞提供重要的营养物质，如肌醇，胆碱和磷酸盐。这一代谢途径在S.酿酒酵母细胞生理学正在探索中。
Phospholipid deacylation results in the formation of glycerophosphodiesters and free fatty acids. In Saccharomyces cerevisiae, four gene products with phospholipase B (deacylating) activity have been characterized (PLB1, PLB2, PLB3, NTE1), and those activities account for most, if not all, of the glycerophosphodiester production observed to date. The glycerophosphodiesters themselves are hydrolyzed into glycerol-3-phosphate and the corresponding alcohol by glycerophosphodiester phosphodiesterases. Although only one glycerophosphodiester phosphodiesterase-encoding gene (GDE1) has been characterized in S. cerevisiae, others certainly exist. Both internal and external glycerophosphodiesters (primarily glycerophosphocholine and glycerophosphoinositol) are formed as a result of phospholipid turnover in S. cerevisiae. A permease encoded by the GIT1 gene imports extracellular glycerophosphodiesters across the plasma membrane, where their hydrolytic products can provide crucial nutrients such as inositol, choline, and phosphate to the cell. The importance of this metabolic pathway in various aspects of S. cerevisiae cell physiology is being explored.