Practical application of aqueous two-phase partition to process development for the recovery of biological products
Practical application of aqueous two-phase partition to process development for the recovery of biological products
复制标题
DOI：
10.1016/j.jchromb.2004.01.008
复制
发表时间：
2004-07-25
影响因子：
3
通讯作者：
Rito-Palomares, M
中科院分区：
文献类型：
--
作者：
Rito-Palomares, M
双水相系统（ATPS）在工艺开发中的实际应用已经开发了几年，用于回收生物产品。不幸的是，这并没有导致该技术在商业过程中的广泛存在。这种情况的一些主要确定的原因涉及的机制的充分理解相形成和行为的溶质分配在ATPS过程中，相形成聚合物的成本和必要的延长的时间来了解工艺开发的技术。在这篇评论文章中，一些实际的缺点归因于ATPS的解决。开发的实际方法来设计ATPS过程中，应用程序实现过程集成，越来越多的使用回收高价值的产品和替代低成本ATPS的最新发展，进行了讨论。建议ATPS工艺应用于生物产品回收的潜在趋势将涉及具有医疗应用的高价值生物颗粒产品的回收。ATPS应用的这一拟议趋势将解决使用可规模化和高效的生物工艺技术快速经济地将新生物制药产品推向市场的迫切需求。(C)2004 Elsevier B.V.保留所有权利。
The practical application of aqueous two-phase systems (ATPS) to process development has been exploited for several years for the recovery of biological products. Unfortunately, this has not resulted in an extensive presence of the technique in commercial processes. Some of the main identified reasons for such situation involve the full understanding of the mechanism governing phase formation and the behaviour of solute partitioning in ATPS processes, the cost of phase forming polymers and the necessary extended time to understand the technique for process development. In this review paper, some of the practical disadvantages attributed to ATPS are addressed. The practical approach exploited to design ATPS processes, the application to achieve process integration, the increasing use for the recovery of high-value products and the recent development of alternative low cost ATPS, are discussed. It is proposed that the potential trend in the application of ATPS processes for the recovery of biological products will involve the recovery of high-value bio-particulate products with medical applications. This proposed trend in the application of ATPS will address the urgent need to rapidly and economically bring new biopharmaceutical products to market using scaleable and efficient bioprocess technology. (C) 2004 Elsevier B.V. All rights reserved.