Comparison of outer-membrane protein subtypes and biotypes of isolates of Haemophilus influenzae type b.
Comparison of outer-membrane protein subtypes and biotypes of isolates of Haemophilus influenzae type b.
复制标题
b型流感嗜血杆菌分离株外膜蛋白亚型和生物型的比较。
DOI：
10.1093/infdis/144.5.480
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Granoff,DM
中科院分区：
文献类型：
--
作者：
Barenkamp,SJ;MunsonJr,RS;Granoff,DM
B型流感嗜血杆菌分离株可通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定其外膜蛋白模式的差异进行亚型划分[1]。这种亚型分型方法已被证明在流行病学研究中很有用，最近扩展到包括21种不同的亚型[3]。Kilian描述了一种不同的嗜血杆菌种属亚分类方法，该方法基于生物化学确定的生物型[2]。在本研究中，我们比较了两个亚分类系统。生物型I占89%的256个菌株测试。未观察到生物型III和V。外膜蛋白的分析允许生物型-I菌株被细分为几个不同的类别。亚型IH是最常见的（44070），其余的生物型I菌株大多有其他常见的亚型。生物型-II和-IV菌株中观察到几乎完全亚型-3L菌株或很少遇到的亚型之间的菌株。当分离株按亚型分为以下类别时，生物型I菌株和生物型I以外的菌株之间亚型分布的差异非常显著：（1）3L以外的常见亚型，（2）3L亚型，和（3）不常见亚型（X2= 79.1;两个自由度; P<10 '8）。
Isolates of Haemophilus influenzae type b may be subtyped by differences in the patterns of their outer-membrane proteins as determined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis [1]. This subtyping method has proven useful in epidemiologic studies and was recently expanded to include 21 different subtypes [3]. Kilian has described a different method for the subclassification of Haemophilus species, which is based on biochemically determined biotypes [2]. In the present study we compared the two subclassification systems. Biotype I accounted for 89% of the 256 isolates tested. Biotypes III and V were not observed. Analysis of outer-membrane proteins permitted biotype-I strains to be subdivided into several different categories. Subtype IH was the most common (44070), and most of the remaining biotype-I strains had other common subtypes. Biotype-Il and-IV strains were observed almost exclusively among subtype-3L strains or among strains with rarely encountered subtypes. The difference in the distribution of subtypes among biotype-I strains and strains other than biotype I was highly significant when isolates were grouped by subtype into the following categories:(1) common subtypes other than 3L,(2) subtype 3L, and (3) uncommon subtypes (X2= 79.1; two degrees of freedom; P< 10'8).