Colonization, ALP activity and plant growth promotion of native and exotic arbuscular mycorrhizal fungi at low pH
Colonization, ALP activity and plant growth promotion of native and exotic arbuscular mycorrhizal fungi at low pH
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低pH条件下本地和外来丛枝菌根真菌的定殖、ALP活性和植物生长促进
DOI：
10.1016/j.soilbio.2006.11.006
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发表时间：
2007-04
影响因子：
9.7
通讯作者：
Yao, Qing
中科院分区：
文献类型：
--
作者：
Zhu, Hong-Hui;Sun, Xiao-Tang;Hu, You-Li;Yao, Qing
土壤酸度对丛枝真菌的影响是多方面的。本研究通过接种白三叶(Trifolium riens L.)用丛枝菌根真菌在两个pH水平(pH 5.0和6.0)下，研究了低pH值对丛枝菌根真菌定殖率、碱性磷酸酶(ALP)活性和促进作用的影响，并比较了原生丛枝菌根真菌群落和外来物种(Gigaspora Margarita)对低pH值的不同反应。PCR-DGGE图谱显示，低pH和高pH条件下原生AMF群落结构存在明显差异。与低pH相比，高pH降低了原生AMF的总定殖率、泡囊定殖率和菌丝外长密度，但对外来AMF的影响不大。无论是本地的还是外来的AMF，高pH都能增加丛枝和真菌结构在定植根中的比例，并具有ALP活性。无论是本地的还是外来的AMF都能促进地上部生物量的增加，且在低pH下的增加幅度大于在高pH下的增加。在两个pH水平下，植物生长对外来AMF的依赖程度均大于对本地AMF的依赖程度。磷的吸收与生物量的变化趋势相同。我们的研究表明，在本实验中，pH的升高对酸性土壤中原生的AMF菌种在生态(群落结构)、功能(ALP活性)和发育(菌丝长度、泡囊和丛枝百分率、总定殖率)方面都有抑制作用，而对外来菌种没有影响。
Soil acidity can affect arbuscular mucorrhizal fungi (AMF) in many aspects. In this study, by inoculating white clover (Trifolium ripens L.) with AMF at two pH levels (pH 5.0 and 6.0), we investigated the influences of low pH on the colonization, alkaline phosphatase (ALP) activity and promoting effect of AMF, and also compared the different responses of native AMF community and exotic species (Gigaspora margarita) to low pH. PCR-DGGE profiles revealed the obvious difference in native AMF community structure between low and high pH. Compared to low pH, high pH decreased the total and vesicle colonization, external hyphae length density of native AMF, but exerted no effect on those of exotic AMF. For both native and exotic AMF, high pH enhanced the proportions of arbuscule and fungal structure with ALP activity in colonized roots. Both native and exotic AMF promoted the shoot biomass, with greater increase at low pH than at high pH. Plant growth was more dependent on exotic AMF than on native AMF at both pH levels. The same trends were observed in P uptake as in biomass. Our study suggested that the increase in pH can exert inhibitory effects on AMF species native to acidic soil, including ecological (community structure), functional (ALP activity) and developmental (hyphal length, vesicle and arbuscule percentage, total colonization) aspects, but have no effect on exotic species in the present experiment.
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影响因子：
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