SAD-3, a Putative Helicase Required for Meiotic Silencing by Unpaired DNA, Interacts with Other Components of the Silencing Machinery.
SAD-3, a Putative Helicase Required for Meiotic Silencing by Unpaired DNA, Interacts with Other Components of the Silencing Machinery.
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DOI：
10.1534/g3.111.000570
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Shiu PK
中科院分区：
文献类型：
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作者：
Hammond TM;Xiao H;Boone EC;Perdue TD;Pukkila PJ;Shiu PK
在粗糙脉孢菌中，减数分裂期间缺乏配对伴侣的基因被称为未配对DNA（MSUD）的减数分裂沉默过程抑制。为了鉴定新的MSUD成分，我们开发了一种高通量的反向遗传筛选，用于N。crassa敲除文库。在这里，我们描述了筛选方法和随后发现的基因（SAD-3）的特性。SAD-3是MSUD和有性孢子产生所需的推定解旋酶。它与其他已知的MSUD蛋白在核周区域（减数分裂沉默活性的中心）中存在于复合物中。SAD-3的直系同源物包括粟酒裂殖酵母Hrr 1，一种RNAi诱导的异染色质形成所需的解旋酶。SAD-3和Hrr 1都与RNA指导的RNA聚合酶和Argonaute相互作用，这表明沉默复合物形成的某些方面可能在两种真菌物种之间是保守的。
In Neurospora crassa, genes lacking a pairing partner during meiosis are suppressed by a process known as meiotic silencing by unpaired DNA (MSUD). To identify novel MSUD components, we have developed a high-throughput reverse-genetic screen for use with the N. crassa knockout library. Here we describe the screening method and the characterization of a gene (sad-3) subsequently discovered. SAD-3 is a putative helicase required for MSUD and sexual spore production. It exists in a complex with other known MSUD proteins in the perinuclear region, a center for meiotic silencing activity. Orthologs of SAD-3 include Schizosaccharomyces pombe Hrr1, a helicase required for RNAi-induced heterochromatin formation. Both SAD-3 and Hrr1 interact with an RNA-directed RNA polymerase and an Argonaute, suggesting that certain aspects of silencing complex formation may be conserved between the two fungal species.