A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita.
A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita.
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DOI：
10.3389/fmicb.2022.870658
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发表时间：
2022
影响因子：
5.2
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中科院分区：
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鸟苷三磷酸(GTP)环水解酶I(GCH1)是四氢生物蝶呤(BH4)合成途径的限制性酶。尽管到目前为止，担子菌中gch1基因的功能还没有被破译，但gch1基因的破坏可能由于微生物中叶酸的营养缺乏而导致条件性死亡。在本研究中，利用RNAi方法下调了绿色木霉(Cyclocybe aegerita，cagch1)中gch1的表达，导致其在固体和液体中的生长受阻，菌丝顶端表现出比野生菌株更多的分枝。突变株的子实体发育明显受阻，菌柄较短，呈瓶状。转录图谱显示，MAPK途径的基因可能参与了cagch1基因敲除所引起的这些效应的调节，这为研究gch1在担子菌发育过程中的作用提供了机会。
Guanosine triphosphate (GTP) cyclohydrolase I (GCH1) is the limiting enzyme of the tetrahydrobiopterin (BH4) synthesis pathway. The disruption of gch1 gene may cause conditional lethality due to folic acid auxotrophy in microorganisms, although the function of gch1 in basidiomycetes has not been deciphered so far. In the present study, gch1 expression in Cyclocybe aegerita (cagch1) was downregulated using the RNAi method, which resulted in growth retardation in both solid and liquid medium, with the hyphal tips exhibiting increased branching compared to that in the wild strain. The development of fruiting bodies in the mutant strains was significantly blocked, and there were short and bottle-shaped stipes. The transcriptional profile revealed that the genes of the MAPK pathway may be involved in the regulation of these effects caused by cagch1 knockdown, which provided an opportunity to study the role of gch1 in the development process of basidiomycetes.