A customized Raman system for point-of-care detection of arthropathic crystals in the synovial fluid.
A customized Raman system for point-of-care detection of arthropathic crystals in the synovial fluid.
复制标题
定制的拉曼系统，用于即时检测滑液中的关节病变晶体。
DOI：
10.1039/c3an02062b
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Akkus,Ozan
中科院分区：
文献类型：
--
作者：
Li,Bolan;Yang,Shan;Akkus,Ozan
尿酸一钠(MSU)和二水焦磷酸钙(CPPD)是关节间隙中最常见的晶体，导致疼痛的关节病。对晶体特性的正确诊断对于适当的疗程至关重要。在这项工作中，定制的拉曼装置与一种实用而高效的样品制备方法相结合，用于MSU和CPPD晶体的自动化学选择性诊断。这些样品是通过对滑液进行简单的酶消化处理，然后进行定制的过滤过程来制备的，该过程能够在亚毫米大小的斑点上聚集晶体。数据采集和收集是自动化的，以收集分布在滤点上的多个光谱。将高性价比拉曼系统的性能与研究级高保真拉曼仪器进行了比较。定制的拉曼装置可以检测出亚临床浓度为0.1μg mLCPPD晶体和1μg mLCPPD晶体的−1。这种实用的样品制备方法与低成本的定制拉曼设备相结合，有可能成为一种新的工具，用于在护理点对关节液中的关节炎晶体进行点击式拉曼诊断。
Monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) are the most frequently observed crystals in joint space, leading to painful arthropathies. Correct diagnosis of the crystal identity is critical for the appropriate course of treatment. In this work, a custom Raman device in combination with a practical and efficient sample preparation method is used for chemically selective diagnosis of MSU and CPPD crystals in an automated fashion. The samples were prepared by brief enzymatic digestion treatment of synovial fluid followed by a customized filtration process which was able to congregate crystals over a submillimeter sized spot. The data acquisition and collection were automated to collect multiple spectra distributed over the filtration spot. The performance of the cost-efficient Raman system was compared to a research-grade high fidelity Raman instrument. The custom-designed Raman device could detect MSU crystals at sub-clinical concentrations of 0.1 μg mL−1 and 1 μg mL−1 for CPPD crystals. This practical sample preparation approach in tandem with the low-cost customized Raman device has potential to be a novel tool for point-and-shoot Raman diagnosis of arthritic crystals in synovial fluid at the point of care.