TRANSFERRIN RECEPTORS IN THE HUMAN GASTROINTESTINAL-TRACT - RELATIONSHIP TO BODY IRON STORES
TRANSFERRIN RECEPTORS IN THE HUMAN GASTROINTESTINAL-TRACT - RELATIONSHIP TO BODY IRON STORES
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DOI：
10.1016/0016-5085(86)90687-6
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发表时间：
1986-10-01
期刊：
影响因子：
29.4
通讯作者：
VALBERG, LS
中科院分区：
文献类型：
--
作者：
BANERJEE, D;FLANAGAN, PR;VALBERG, LS
免疫标记的抗体用于识别转铁蛋白受体和粘膜转铁蛋白在人胃肠道活检切片。转铁蛋白受体在十二指肠、回肠和结肠的绒毛上皮和隐窝区域中是明显的，主要在基底侧区域。在7名低铁储备的受试者中，受体的十二指肠绒毛染色强度（0-4级）为2.1 ±-。0.3（平均值±）。SD）。该值显著高于13名具有正常铁储存的受试者的值（1.1 ± 0.01）。0.4）。5例遗传性血色素沉着症患者十二指肠转铁蛋白受体染色与铁储备正常者无明显差异。在十二指肠、回肠和结肠上皮细胞的顶端细胞质中发现转铁蛋白染色，但观察者评估的重现性不足以进行定量分析。我们的研究结果表明，铁缺乏是伴随着转铁蛋白受体在十二指肠吸收细胞的增加，和遗传性病变血色素沉着症不涉及转铁蛋白受体在肠粘膜与正常铁储存的受试者相比，增加。
Fluorescently labeled antibodies were used to identify transferrin receptors and mucosal transferrin in human gastrointestinal biopsy sections. Transferrin receptors were evident in the villous epithelium and the crypt areas of duodenum, ileum, and colon, predominantly in the basal-lateral area. In 7 subjects with low iron stores, the intensity of duodenal villous staining for receptor, on a scale of 0-4, was 2.1 .+-. 0.3 (mean .+-. SD). This value was significantly higher than the value in 13 subjects with normal iron stores (1.1 .+-. 0.4). In 5 patients with hereditary hemochromatosis, duodenal transferrin receptor staining was not significantly different from that in the subjects with normal iron stores. Transferrin staining was found in the apical cytoplasm of epithelial cells in the duodenum, ileum, and colon, but observer assessment was not sufficiently reproducible to make a quantitative analysis. Our results suggest that iron deficiency is accompanied by an increase in transferrin receptors in duodenal absorptive cells, and the genetic lesion in hemochromatosis does not involve an increase in transferrin receptors in the intestinal mucosa compared with subjects with normal iron stores.