Dietary l-arginine supplementation enhances intestinal development and expression of vascular endothelial growth factor in weanling piglets
Dietary l-arginine supplementation enhances intestinal development and expression of vascular endothelial growth factor in weanling piglets
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DOI：
10.1017/s000711451000365x
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发表时间：
2011-01
影响因子：
3.6
通讯作者：
K. Yao;S. Guan;Tiejun Li;Ruilin Huang;Guoyao Wu;Z. Ruan;Yulong Yin
中科院分区：
文献类型：
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作者：
K. Yao;S. Guan;Tiejun Li;Ruilin Huang;Guoyao Wu;Z. Ruan;Yulong Yin
据报道，口服L-精氨酸可预防人类婴儿的肠道疾病。然而，关于日粮中添加精氨酸对断奶仔猪肠道发育的影响还知之甚少。选用20头体重5.3（sem 0.13）kg的21日龄去势仔猪，从母猪断奶后，单独圈养，随机分为玉米和豆粕两组，每组10头。饲喂7 d后，每组随机抽取6头猪，取其各种组织。与对照组相比，日粮中添加1%L-精氨酸对采食量无显著影响，但显著提高了小肠相对重量（+33%）、日增重（+38%）和饲料效率（+28%）（P <0.05）。结果表明，添加甘氨酸的仔猪十二指肠、空肠和回肠的绒毛高度分别比不添加甘氨酸的对照组高21%、28%和25%（P <0.05）。补充精氨酸使十二指肠、空肠和回肠粘膜血管内皮生长因子（VEGF）蛋白水平分别增加14%、39%和35%（P <0.05）。与对照组相比，在膳食中补充1%l-精氨酸增加了（P <0.05）精氨酸和胰岛素（+36%）的血浆浓度，降低了（P <0.05）皮质醇（-33%），NH3（-21%）和尿素（-19%）的血浆浓度。这些结果表明，精氨酸补充增强肠道生长，发育和表达VEGF的早期断奶仔猪喂养玉米和大豆粉为基础的饮食。这些发现可能对处于压力或疾病条件下的新生猪具有重要意义。
Oral administration of l-arginine has been reported to prevent gut disease in human infants. However, little is known about the effects of dietary arginine supplementation on intestinal development of weaned piglets. In the present study, twenty 21-d-old castrated piglets with 5·3 (sem 0·13) kg body weight (BW) were weaned from sows, individually housed and randomly assigned to one of the two maize- and soyabean meal-based diets supplemented with 0 or 1 % l-arginine. After consuming the diets for 7 d, six pigs were randomly selected from each group to obtain various tissues. Compared with control pigs, dietary supplementation with 1 % l-arginine did not affect feed intake but enhanced (P < 0·05) the relative weight of the small intestine (+33 %), daily BW gain (+38 %) and feed efficiency (+28 %). The villus height of the duodenum, jejunum and ileum in arginine-supplemented piglets was 21, 28 and 25 % greater (P < 0·05) than in the non-supplemented control group. Arginine supplementation increased (P < 0·05) protein levels for vascular endothelial growth factor (VEGF) in duodenal, jejunal and ileal mucosae by 14, 39 and 35 %, respectively. Compared with the control group, dietary supplementation with 1 % l-arginine increased (P < 0·05) plasma concentrations of arginine and insulin (+36 %), and decreased (P < 0·05) plasma concentrations of cortisol ( − 33 %), NH3 ( − 21 %) and urea ( − 19 %). These results indicate that arginine supplementation enhances intestinal growth, development and expression of VEGF in early-weaned pigs fed a maize- and soyabean meal-based diet. The findings may have important implications for neonatal pigs under stressful or diseased conditions.