Involvement of a replicative DNA helicase of bacteriophage T4 in DNA recombination.
Involvement of a replicative DNA helicase of bacteriophage T4 in DNA recombination.
复制标题
噬菌体 T4 的复制 DNA 解旋酶参与 DNA 重组。
DOI：
10.1093/genetics/138.2.247
复制
发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Yonesaki,T
中科院分区：
文献类型：
--
作者：
Yonesaki,T
噬菌体T4基因41编码一种复制型DNA解旋酶，它是对滞后链DNA合成必不可少的原始体的一个亚基。一个突变，rrh，产生和选择的解旋酶基因的基础上有限的DNA复制，提前停止。rrh降低了紫外线照射噬菌体的存活率和基因重组的频率。此外，rrh减少了多联体DNA的产生。这些结果有力地表明，基因41复制解旋酶参与DNA重组。
Bacteriophage T4 gene 41 encodes a replicative DNA helicase that is a subunit of the primosome which is essential for lagging-strand DNA synthesis. A mutation, rrh, was generated and selected in the helicase gene on the basis of limited DNA replication that ceases early. The survival of ultraviolet-irradiated phage and the frequency of genetic recombination are reduced by rrh. In addition, rrh diminishes the production of concatemeric DNA. These results strongly suggest that the gene 41 replicative helicase participates in DNA recombination.