Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)
Analytical sensitivity of rapid influenza antigen detection tests for swine-origin influenza virus (H1N1)
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DOI：
10.1016/j.jcv.2009.05.034
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发表时间：
2009-07-01
影响因子：
8.8
通讯作者：
Peiris, J. S. M.
中科院分区：
文献类型：
--
作者：
Chan, K. H.;Lai, S. T.;Peiris, J. S. M.
背景资料：一种新型猪源流感病毒（S-OIV）（H1N1）正在全球范围内传播，并有成为大流行的威胁。目的：确定选定的市售快速流感抗原检测试验在检测S-OIV H1N1中的分析灵敏度。两种S-OIV分离株的系列稀释液，一个季节性甲型H1N1流感病毒分离株和一个来自S-在五种市售流感抗原检测试验中并通过细胞培养中的病毒分离来检测OIV病。结果：5种流感病毒抗原检测方法对S-OIV的灵敏度分别为99.9%、100.0%、1000.0%、100.0%、10.0%、10%（组织培养感染剂量50（TCID 50）log（10）3.3-4.7，与季节性流感相当结论：所选甲型流感抗原检测试剂检测S-IOV的分析灵敏度与季节性H1N1流感的分析灵敏度相当。(C)2009爱思唯尔有限公司版权所有。
Background: A novel swine origin influenza virus (S-OIV) (H1N1) is spreading worldwide and threatens to become pandemic.Objectives: Determine analytical sensitivity of selected commercially available rapid influenza antigen detection tests in detecting S-OIV H1N1.Study design: Serial dilutions of two S-OIV isolates, one seasonal influenza A (H1N1) isolate and a nasopharyngeal aspirate from a patient with S-OIV disease were tested in five commercially available influenza antigen detection tests and by virus isolation in cell culture. Viral M gene copy number was determined by quantitative PCR methods.Results: The analytical sensitivity of the five influenza antigen detection tests for S-OIV (tissue culture infectious dose 50 (TCID50) log(10) 3.3-4.7 was comparable with that of seasonal influenza (TCID50 log(10) 4.0-4.5).Conclusion: The analytical sensitivity of the selected influenza A antigen detection tests for detection of S-IOV was comparable with that of seasonal influenza H1N1. (C) 2009 Elsevier B.V. All rights reserved.