Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis.
Lineage-Specific Profiling Delineates the Emergence and Progression of Naive Pluripotency in Mammalian Embryogenesis.
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DOI：
10.1016/j.devcel.2015.10.011
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发表时间：
2015-11-09
影响因子：
11.8
通讯作者：
Bertone P
中科院分区：
文献类型：
--
作者：
Boroviak T;Loos R;Lombard P;Okahara J;Behr R;Sasaki E;Nichols J;Smith A;Bertone P
原始多能性在哺乳动物胚胎着床前表现明显。在这里，我们使用谱系特异性RNA测序来确定小鼠从8细胞阶段发育到移植后的转录组动力学。该方法结合了高灵敏度和基于报告者的命运分配，从离散的胚胎细胞类型中获得基因表达的全谱。我们定义了指示发育状态和时间调节模式的表达模块，标志着体内初始多能性的建立和解除。对胚胎干细胞和滞育胚胎的分析表明，着床前外胚层的核心转录回路几乎完全保存。与狨猴灵长类动物囊胚的内细胞团比较，发现了类似的多能性因子补充，但使用了不同的信号通路。胚胎培养实验进一步表明，与啮齿类动物不同，狨猴胚胎在早期谱系分离过程中利用WNT信号。这些发现支持了原始多能性的保守转录因子基础，同时揭示了谱系分离的物种特异性调控特征。转录组分析为小鼠早期发育建立了一个参考图谱谱系特异性RNA-seq捕获基因表达的完整动态范围ESCs和滞育和着床前外胚层具有共同的多能性回路灵长类动物早期ICM利用额外的信号通路进行谱系说明在这篇资源文章中，Boroviak等人对早期小鼠，滞育和狨猴胚胎进行了谱系特异性转录组分析。定义调控模块介导幼稚多能性的建立和丧失。跨物种比较揭示了保守的多能性转录网络，但内部细胞团分离的信号通路不同。
Naive pluripotency is manifest in the preimplantation mammalian embryo. Here we determine transcriptome dynamics of mouse development from the eight-cell stage to postimplantation using lineage-specific RNA sequencing. This method combines high sensitivity and reporter-based fate assignment to acquire the full spectrum of gene expression from discrete embryonic cell types. We define expression modules indicative of developmental state and temporal regulatory patterns marking the establishment and dissolution of naive pluripotency in vivo. Analysis of embryonic stem cells and diapaused embryos reveals near-complete conservation of the core transcriptional circuitry operative in the preimplantation epiblast. Comparison to inner cell masses of marmoset primate blastocysts identifies a similar complement of pluripotency factors but use of alternative signaling pathways. Embryo culture experiments further indicate that marmoset embryos utilize WNT signaling during early lineage segregation, unlike rodents. These findings support a conserved transcription factor foundation for naive pluripotency while revealing species-specific regulatory features of lineage segregation. Transcriptome profiling establishes a reference map for early mouse development Lineage-specific RNA-seq captures the full dynamic range of gene expression ESCs and diapaused and preimplantation epiblasts share common pluripotency circuitry Primate early ICM utilizes additional signaling pathways for lineage specification In this Resource article, Boroviak et al. perform lineage-specific transcriptome analysis of early mouse, diapaused, and marmoset embryos, defining regulatory modules mediating the establishment and loss of naive pluripotency. Cross-species comparisons reveal a conserved pluripotency transcriptional network but distinct signaling pathways for segregation of the inner cell mass.