Molecular Characterization of Nine Tissue-Specific or Stress-Responsive Genes of Histone Deacetylase in Tomato (Solanum lycopersicum)
Molecular Characterization of Nine Tissue-Specific or Stress-Responsive Genes of Histone Deacetylase in Tomato (Solanum lycopersicum)
复制标题
番茄（Solanum lycopersicum）中九个组织特异性或应激反应性组蛋白脱乙酰酶基因的分子特征
DOI：
10.1007/s00344-016-9660-8
复制
发表时间：
2017-09-01
影响因子：
4.8
通讯作者：
Chen, Guoping
中科院分区：
文献类型：
--
作者：
Guo, Jun-E;Hu, Zongli;Chen, Guoping
组蛋白乙酰化和去乙酰化通过染色质修饰在植物生长发育中发挥重要作用。在不同的组织和发育阶段，组蛋白乙酰化和去乙酰化的调控是由组蛋白乙酰化转移酶和组蛋白去乙酰化酶(HDACs)控制的。关于基因组稳定性、转录调控、发育和对逆境反应的重要性的知识已经从模式植物拟南芥中获得。然而，关于番茄的生物学功能和发育或与胁迫相关的HDAC基因的信息很少。本研究从SLHDA1-SLHDA9中克隆了9个番茄组蛋白脱乙酰酶基因RPD3/HDA1亚家族基因，它们编码的组蛋白脱乙酰酶蛋白与已知的植物HDACs具有很高的相似性和保守结构域。根据系统发育分析，将这些HDAC基因进一步细分为四类，即I类、II类、III类和IV类。定量RT-PCR分析表明，9个SLHDAC基因在不同转录物丰度的组织中均有表达，并且表现出不同的组织特异性表达谱，这表明它们可能在番茄的生长发育中发挥着关键而不同的作用。在不同的非生物胁迫下，包括盐胁迫、脱水胁迫和高/低温胁迫下，这些SLHDACs的转录水平都有不同程度的诱导。这9个SLHDAC是由上述不同或相似诱导水平的应激诱导的。总之，本研究为进一步探索HDAC基因在番茄发育和果实成熟过程中的调控以及对环境胁迫的响应提供了有价值的信息。
Histone acetylation and deacetylation play an important role in plant growth and development by chromatin modifications. Regulation of histone acetylation and deacetylation is controlled by histone acetyltransferases and histone deacetylases (HDACs) in different tissues and development stages. Knowledge of the importance of genome stability, transcriptional regulation, development and response to stress has been obtained from the model plantArabidopsis. However, little information on biological functions and development or stress-relatedHDACgenes is available in tomato. In this study, nine tomato histone deacetylase genes of the RPD3/HDA1 subfamily, fromSlHDA1-SlHDA9, were characterized to encode histone deacetylase proteins that share high similarity of protein sequences and conserved domains with those of known plant HDACs. TheseHDACgenes have been further subdivided into four groups, namely Class I, Class II, Class III and Class IV based on phylogenetic analysis. Quantitative RT-PCR analysis revealed that the nineSlHDACgenes were expressed in all tissues with different transcript abundance and exhibited different tissue-specific expression profiles, suggesting that they may have crucial and diverse roles in tomato growth and development. Varying degrees of induction were detected in the transcript levels of theseSlHDACsunder various abiotic stresses including NaCl, dehydration, and high/low temperature. These nineSlHDACswere induced by the above stresses with differential/similar induction levels. Overall, this study provides valuable information for further exploring the regulation ofHDACgenes during tomato development and fruit ripening and in response to environmental stresses.