Direct fermentation of newspaper after laccase-treatment using yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase
Direct fermentation of newspaper after laccase-treatment using yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase
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DOI：
10.1016/j.renene.2012.01.078
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发表时间：
2012-08-01
期刊：
影响因子：
8.7
通讯作者：
Ueda, Mitsuyoshi
中科院分区：
文献类型：
--
作者：
Nakanishi, Akihito;Kuroda, Kouichi;Ueda, Mitsuyoshi
酿酒酵母不能利用纤维素作为碳源。在自然界中，纤维素主要被3种纤维素酶降解为葡萄糖：内切葡聚糖酶II（EG）、纤维二糖水解酶II（CBH）和β-葡糖苷酶I（BC）。利用细胞表面工程技术构建了共表达这3种酶的酵母菌，并将其用于直接发酵纤维素生产乙醇。利用构建的酵母菌直接发酵碳源报纸。首先用Trametes sp. Ha 1漆酶对报纸进行预处理，然后用漆酶处理后的报纸考察构建的酵母菌的发酵情况。结果表明，经漆酶预处理的废纸可以作为一种资源，利用共展示EC、CBH和BC的酵母菌直接发酵生产乙醇。(C)2012爱思唯尔有限公司保留所有权利。
Saccharomyces cerevisiae cannot utilize cellulose as a carbon source. In nature, cellulose is mainly degraded into glucose by 3 cellulases: endoglucanase II (EG), cellobiohydrolase II (CBH), and beta-glucosidase I (BC). A yeast codisplaying these 3 enzymes was constructed by cell surface engineering to directly ferment cellulose into ethanol. The constructed yeast was used to directly ferment newspaper-a carbon source. The newspaper was first pretreated with Trametes sp. Ha1 laccase, and the laccase-treated newspaper was used for investigating the fermentation by the constructed yeast. The results indicated that the laccase-pretreated waste paper could serve as a resource for the production of ethanol by direct fermentation mediated by yeast codisplaying EC, CBH, and BC. (C) 2012 Elsevier Ltd. All rights reserved.