An inactive, prorenin-like substance in human kidney and plasma.
An inactive, prorenin-like substance in human kidney and plasma.
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人类肾脏和血浆中的一种无活性的肾素原样物质。
DOI：
10.1042/cs059029s
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Hesson,TE
中科院分区：
文献类型：
--
作者：
Atlas,SA;Laragh,JH;Sealey,JE;Hesson,TE
1.血浆原肾素（非活性肾素）约占人血浆中总肾素的70%，通过Cibacron blue F3 G-A-agarose亲和层析，几乎完全与活性肾素分离。在伴刀豆球蛋白A-Sepharose上可以除去存在于原肾素峰中的轻微残留的肾素活性，表明原肾素完全无活性。2.用胰蛋白酶孵育后，人肾皮质提取物和肾灌流液的肾素活性均增加。这种胰蛋白酶可激活的肾素分别占提取物和灌流液中总肾素的15%和40%。3.与血浆前肾蛋白一样，来自肾脏提取物和肾脏灌流液的胰蛋白酶活化的肾蛋白酶几乎完全与汽巴蓝F3 G-A-琼脂糖上的活性肾蛋白酶分离。经过额外的色谱步骤，胰蛋白酶激活的肾素从肾皮质提取物被发现是完全无活性的。4.我们的结论是，人的肾脏含有，并能够释放，胰蛋白酶激活的肾素，类似于血浆原肾素。它可能不同于许多60 000分子量形式的肾素先前确定在肾提取物中，因为这些具有相当大的内在肾素活性，并可能代表一个复合物的肾素与结合蛋白。
1. Plasma prorenin (inactive renin), which accounts for about 70% of the total renin in human plasma, was almost completely separated from active renin by affinity chromatography on Cibacron blue F3G-A-agarose. The slight residual renin activity present in the prorenin peak can be removed on concanavalin A-Sepharose, demonstrating that prorenin is completely inactive. 2. The renin activity of both human renal cortical extract and renal perfusate increased after incubation with trypsin. This trypsin-activable renin accounted for 15 and 40% of the total renin in extract and perfusate respectively. 3. Trypsin-activable renin from both renal extract and renal perfusate was, like plasma prorenin, almost completely separated from active renin on Cibacron blue F3G-A-agarose. After additional chromatographic steps, the trypsin-activable renin from renal cortical extract was found to be completely inactive. 4. We conclude that human kidney contains, and is able to release, a trypsin-activable renin that resembles plasma prorenin. It may differ from many of the 60 000 molecular-weight forms of renin previously identified in renal extracts, since these possess considerable intrinsic renin activity and probably represent a complex of renin with a binding protein.