Ipomoea obscura ameliorates cyclophosphamide-induced toxicity by modulating the immune system and levels of proinflammatory cytokine and GSH
Ipomoea obscura ameliorates cyclophosphamide-induced toxicity by modulating the immune system and levels of proinflammatory cytokine and GSH
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DOI：
10.1139/y10-086
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发表时间：
2010-11-01
影响因子：
2.1
通讯作者：
Kuttan, Girija
中科院分区：
文献类型：
--
作者：
Hamsa, T. P.;Kuttan, Girija
牵牛花是一种被广泛使用的药用植物。本研究的目的是在小鼠模型中研究其对环磷酰胺（CTX）诱导的毒性的保护活性。瑞士白化病小鼠腹腔注射CTX（25 mg/kg体重）沿着I. obscura提取物（10 mg/kg体重）持续10天。提取物显著降低了CTX引起的骨髓抑制，并改善了相对器官重量、总白色血细胞计数和骨髓细胞构成。与肝脏病理生理学相关的参数水平升高，即谷氨酸丙酮酸转氨酶，碱性磷酸酶和脂质过氧化，提取物治疗显着降低。CTX处理的动物的肝脏和肠道谷胱甘肽水平的降低被I逆转。暗房研究发现，在I中，CTX治疗后降低的细胞因子（即IFN-γ、IL-2和粒细胞-单核细胞集落刺激因子）水平有所增加。Obscura治疗的动物。用I. obscura还可以降低促炎细胞因子TNF-α的水平。数据显示，I. Obscura可作为有效的化学保护剂，并可用作化学治疗应用中的佐剂。
Ipomoea obscura L. is a widely used medicinal plant. The objective of this study was to investigate its protective activity against cyclophosphamide (CTX)-induced toxicity in mouse models. Swiss albino mice were treated intraperitoneally with CTX (25 mg/kg body weight) along with I. obscura extract (10 mg/kg body weight) for 10 days. Extract significantly reduced myelosuppression caused by CTX and improved the relative organ weight, total white blood cell count, and bone marrow cellularity. The elevated levels of parameters related to pathophysiology of the liver, namely glutamate pyruvate transaminase, alkaline phosphatase, and lipid peroxidation, were significantly reduced by extract treatment. Reduction of liver and intestinal glutathione levels of CTX-treated animals was reversed by I. obscura. The lowered levels of cytokines, namely IFN-gamma, IL-2, and granulocyte-monocyte colony-stimulating factor after CTX treatment were found to be increased in I. obscura treated animals. Treatment with I. obscura could also decrease the level of proinflammatory cytokine TNF-alpha. The data suggested that I. obscura can act as a potent chemoprotective agent and can be used as an adjuvant in chemotherapeutic applications.