Lipid productivity and cell wall ultrastructure of six strains of Nannochloropsis: Implications for biofuel production and downstream processing
Lipid productivity and cell wall ultrastructure of six strains of Nannochloropsis: Implications for biofuel production and downstream processing
复制标题
DOI：
10.1016/j.algal.2014.09.003
复制
发表时间：
2014-10-01
影响因子：
5.1
通讯作者：
Ali, Sohail T.
中科院分区：
文献类型：
--
作者：
Beacham, Tracey A.;Bradley, Claire;Ali, Sohail T.
微藻对第三代生物柴油的生产产生了相当大的兴趣。然而，由于即使在被认为形态相似的菌株中也观察到细胞生理学、代谢潜力和遗传学的显著变化，因此适当的菌株选择被证明具有挑战性。评估来自CCAP培养物收集的六株微拟球藻的脂质生产力和细胞结构，作为油生产和收获容易性的代表，以评估它们作为生物柴油生产平台的适用性。观察到菌株间生长速率和脂质积累的差异。与微拟球藻菌株849/3相比，微拟球藻菌株849/7显示出显著减少的倍增时间，而海洋微拟球藻849/10产生最高的脂质含量。此外，6个菌株可以根据其细胞壁厚度分为3个不同的类别，其在菌株之间从63至119 nm变化，并且与物种和地理分离位置无关。这些变化在生物柴油生产的超微结构和生理的重要性进行了讨论。(C)2014爱思唯尔有限公司版权所有。
Microalgae are generating considerable interest for third generation biodiesel production. However, appropriate strain selection is proving challenging due to the significant variation in cellular physiology, metabolic potential and genetics observed even amongst strains deemed morphologically similar. Six strains of Nannochloropsis from the CCAP culture collection were assessed for their lipid productivity and cellular structure, as proxies for oil production and harvesting ease, to assess their suitability as biodiesel production platforms. Differences in growth rate and lipid accumulation across the strains were observed. Nannochloropsis oculata strain 849/7 showed significantly reduced doubling time compared to Nannochloropsis salina strain 849/3, whilst Nannochloropsis oceanica 849/10 produced the highest lipid content. In addition the six strains could be differentiated into 3 distinct classes based on their cell wall thickness, which varied across the strains from 63 to 119 nm and which is independent of both species and geographical isolation location. The importance of these variations in ultrastructure and physiology for biodiesel production is discussed. (C) 2014 Elsevier B.V. All rights reserved.