A COMPARISON OF TUMOR AND NORMAL TISSUE MICROVASCULAR HEMATOCRITS AND RED-CELL FLUXES IN A RAT WINDOW CHAMBER MODEL
A COMPARISON OF TUMOR AND NORMAL TISSUE MICROVASCULAR HEMATOCRITS AND RED-CELL FLUXES IN A RAT WINDOW CHAMBER MODEL
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DOI：
10.1016/0360-3016(93)90348-y
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发表时间：
1993-01-15
影响因子：
7
通讯作者：
DEWHIRST, MW
中科院分区：
文献类型：
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作者：
BRIZEL, DM;KLITZMAN, B;DEWHIRST, MW
该实验室此前曾使用窗口小室模型测量F-344大鼠乳腺肿瘤和正常肉芽组织中的红细胞速度。由于红细胞流量和红细胞压积更准确地反映了血液的携氧潜力，因此我们使用该模型来测量这些参数。用异硫氰酸荧光素标记大鼠红细胞，静脉注射0.2ml填充细胞给8~10天R-3230乳腺癌大鼠。β-藻红蛋白(0.15 mg.)也被注射并作为血浆染料来勾勒血管轮廓。然后采集外周血样本，用流式细胞仪进行分析，以确定红细胞的标记分数。将流动的肿瘤和正常组织血管记录在VCR上，并使用这些视频图像来确定血管的长度和直径、红细胞流量和速度以及红细胞压积。肿瘤组织的平均血管直径和对数(红细胞流量)显著大于正常组织(p=0.007和p<；0.025)。在控制了这些变量后，肿瘤组织的红细胞压积的中位数为19%，而正常组织的红细胞压积的中位数为15%，两者差异不显著。这项技术提供了一种无毒和可重复性的方法，目前正被用于辅助体内定义肿瘤的氧合。
This laboratory has previously used a window chamber model to measure red blood cell velocity in mammary tumors and normal granulation tissues of the F-344 rat. Because red cell flux and hematocrit more accurately reflect the oxygen carrying potential of blood, we used this model to measure these parameters. Red blood cells were labelled with fluorescein isothiocyanate, and 0.2 ml. packed cells were injected intravenously into rats bearing an 8 to 10 day old R-3230 mammary carcinoma. Beta-phycoerythrin (0.15 mg.) was also injected and served as a plasma dye to outline the blood vessels. A sample of peripheral blood was then taken and analyzed by flow cytometry to determine the labeled fraction of red blood cells. Flowing tumor and normal tissue vessels were recorded onto a VCR, and these video images were used to determine vascular length and diameter, RBC flux and velocity, and hematocrit. Median vessel diameter and log, (red blood cell flux) were significantly greater in tumors than in normal tissues (p = 0.007 and p < 0.025, respectively). After controlling for these variables, the median tumor hematocrit of 19% was not significantly greater than the median normal tissue hematocrit of 15%. This technique provides a nontoxic and reproducible method that is now being used to assist in the in vivo definition of tumor oxygenation.