Antinociceptive effects of intrathecally administered huwentoxin-I, a selective N-type calcium channel blocker, in the formalin test in conscious rats
Antinociceptive effects of intrathecally administered huwentoxin-I, a selective N-type calcium channel blocker, in the formalin test in conscious rats
复制标题
鞘内注射虎纹鸟毒素-I（一种选择性 N 型钙通道阻滞剂）在清醒大鼠福尔马林试验中的镇痛作用
DOI：
10.1016/j.toxicon.2004.08.018
复制
发表时间：
2005-01-01
期刊：
影响因子：
2.8
通讯作者：
Liang, SP
中科院分区：
文献类型：
--
作者：
Chen, JQ;Zhang, YQ;Liang, SP
本研究以清醒大鼠福尔马林致痛实验为对象，通过比较鞘内注射虎纹捕鸟蛛（Ornithoctonus huwena（Wang）[Selenocosmia huwena wang]）毒N型钙通道阻滞剂虎纹捕鸟蛛毒素-I（HWTX-I）与ω-芋螺毒素-MVIIA（Omega-CTX-MVIIA）和盐酸吗啡的镇痛作用，探讨了HWTX-I的镇痛作用。类似于w-CTX-MVIIA和吗啡，鞘内预处理HWTX-I导致以剂量依赖性方式抑制伤害性行为。HWTX-I和omega-CTX-MVIIA的ED 50值分别为0.28和0.19 μ g/kg。还发现，在较低剂量（0.1和0.5 μ g/kg）下，HWTX-1的抗伤害感受作用与ω-CTX-MVIIA的相同，而ω-CTX-MVIIA在较高剂量（1.0 μ g/kg）下比HWTX-1作用更显著。但omega-CTX-MVIIA的抗伤害作用伴随有运动功能障碍，且随着剂量的增加，这些副作用更加明显。相比之下，HWTX-I在0.5-1.0 μ g/kg剂量下未显示出这些副作用。与HWTX-I和omega-CTX-MVIIA相比，鞘内注射盐酸吗啡的镇痛作用开始更快，但持续时间更短（1.0 μ g/kg时约2-3小时），而HWTX-I和omega-CTX-MVIIA的镇痛作用持续时间更短（1.0 μ g/kg时约4-5小时）。因此，本结果表明，与w-CTX-MVIIA一样，鞘内施用HWTX-I在福尔马林试验的大鼠模型中的抗伤害感受中有效。(C)2004爱思唯尔有限公司保留所有权利。
The present study was undertaken to elucidate the antinociceptive effect of intrathecal administration of huwentoxin-I (HWTX-I), a N-type calcium channel blocker from the venom of the Chinese bird spider Ornithoctonus huwena (Wang) [Selenocosmia huwena wang], by comparison with omega-Conotoxin-MVIIA (omega-CTX-MVIIA) and morphine hydrochloride in the formalin test in conscious rats. Similar to w-CTX-MVIIA and morphine, intrathecal pre-treatment with HWTX-I resulted in suppression of nociceptive behavior in a dose-dependent manner. The ED50 values of HWTX-I and omega-CTX-MVIIA were 0.28 and 0.19 mug/kg, respectively. It was also found that, at lower doses (0.1 and 0.5 mug/kg), the antinociceptive effect of HWTX-I was identical to that of omega-CTX-MVIIA, while omega-CTX-MVIIA acted more remarkably than HWTX-1 at higher dose (1.0 mug/kg). However, the antinociception induced by omega-CTX-MVIIA were companied with motor dysfunction, and these side-effects became more evident with the doses of omega-CTX-MVIIA increasing. In contrast, HWTX-I did not show these side-effects at the doses of 0.5-1.0 mug/kg. Compared with HWTX-I and omega-CTX-MVIIA, the analgesic effect of intrathecal morphine hydrochloride was initiated faster, but lasted for a shorter time (about 2-3 h at 1.0 mug/kg) than that of HWTX-I and omega-CTX-MVIIA (about 4-5 h at 1.0 mug/kg). Therefore, the present results show that, like w-CTX-MVIIA, the intrathecal administration of HWTX-I is effective in antinociception in the rat model of the formalin test. (C) 2004 Elsevier Ltd. All rights reserved.