Molecular cloning of cDNA for lipopolysaccharide-binding protein from the hemolymph of the American cockroach, Periplaneta americana. Similarity of the protein with animal lectins and its acute phase expression.
Molecular cloning of cDNA for lipopolysaccharide-binding protein from the hemolymph of the American cockroach, Periplaneta americana. Similarity of the protein with animal lectins and its acute phase expression.
复制标题
美洲大蠊血淋巴中脂多糖结合蛋白 cDNA 的分子克隆。
DOI：
10.1016/s0021-9258(18)98841-1
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Natori
中科院分区：
文献类型：
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作者：
T. Jomori;S. Natori
以前的一篇论文描述了从美洲大蠊(Jomori，T.，Kubo，T.，和Natori，S.(1990)EUR.)血淋巴中分离纯化一种钙依赖的脂多糖结合蛋白。J.生物化学。190、201-206)。本文介绍了内毒素结合蛋白基因的克隆和鉴定。该蛋白在其羧基末端有一个碳水化合物识别结构域，含有在多种哺乳动物C型凝集素中保守的氨基酸序列。它还含有一个N-连接的碳水化合物链，携带该链的氨基酸残基被指定为56位的天冬氨酸(氨基端的第23个氨基酸残基)。Northern印迹分析表明，在成虫体内注射大肠杆菌后，存在与该基因杂交的多个mRNAs，且其含量短暂增加，提示该蛋白在该虫的急性期反应中起作用。
A previous paper described the purification of a calcium-dependent lipopolysaccharide-binding protein from the hemolymph of Periplaneta americana (Jomori, T., Kubo, T., and Natori, S. (1990) Eur. J. Biochem. 190, 201-206). This paper describes the molecular cloning and characterization of cDNA for the LPS-binding protein. This protein was found to have a carbohydrate-recognition domain at its carboxyl terminus containing amino acid sequences that are conserved in various mammalian C-type lectins. It was also shown to contain an N-linked carbohydrate chain, and the amino acid residue carrying this chain was assigned as Asn at position 56 (23rd amino acid residue from the amino terminus). Northern blot analysis revealed the presence of multiple mRNAs that hybridized with this cDNA and transient increases in their content after injection of Escherichia coli into adult Periplaneta, suggesting that the LPS-binding protein plays a role in the acute phase response of this insect.