Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF).
Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF).
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DOI：
10.1186/s40729-017-0081-7
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发表时间：
2017-12
影响因子：
2.7
通讯作者：
Kawase T
中科院分区：
文献类型：
--
作者：
Isobe K;Watanebe T;Kawabata H;Kitamura Y;Okudera T;Okudera H;Uematsu K;Okuda K;Nakata K;Tanaka T;Kawase T
先进的富血小板纤维蛋白（A-PRF）或浓缩生长因子（CGF）制备的纤维蛋白凝块膜，尽管其生物降解性相对较快，但已被用作牙槽骨组织再生的生物活性屏障膜。随着膜降解，生长因子被认为是逐渐释放的。然而，这些膜的机械性能和可降解性能尚未得到很好的表征。本研究的目的是机械和化学表征这些膜。A-PRF和CGF凝块取自非吸烟健康供者的血液样本，并被压缩成1毫米厚的膜。将牛凝血酶添加到血小板不足的血浆中制备血小板不足血浆源性纤维蛋白（PPTF）凝块。以1 mm/min的速度进行拉伸试验。用扫描电镜观察纤维蛋白纤维的形态。在含有胰蛋白酶和EDTA的PBS中进行消化试验。在拉伸试验中，A-PRF和CGF在杨氏模量、断裂应变或最大应力方面没有观察到统计学差异。在断裂菌株中，PPTF明显弱于CGF。同样，PPTF的纤维蛋白纤维厚度和交联密度小于其他膜，并且PPTF的降解速度比其他膜快。虽然离心条件不同，但A-PRF和CGF的制备机理基本相同。因此，可以想象这两种膜具有相似的机械和化学性质。只有通过不同机制制备的PPTF机械性能较弱，酶降解能力较强。
Fibrin clot membranes prepared from advanced platelet-rich fibrin (A-PRF) or concentrated growth factors (CGF), despite their relatively rapid biodegradability, have been used as bioactive barrier membranes for alveolar bone tissue regeneration. As the membranes degrade, it is thought that the growth factors are gradually released. However, the mechanical and degradable properties of these membranes have not well been characterized. The purpose of this study was to mechanically and chemically characterize these membranes. A-PRF and CGF clots were prepared from blood samples collected from non-smoking, healthy donors and were compressed to form 1-mm-thick membranes. Platelet-poor plasma-derived fibrin (PPTF) clots were prepared by adding bovine thrombin to platelet-poor plasma. A tensile test was performed at the speed of 1 mm/min. Morphology of the fibrin fibers was examined by SEM. A digestion test was performed in PBS containing trypsin and EDTA. In the tensile test, statistical difference was not observed in Young’s modulus, strain at break, or maximum stress between A-PRF and CGF. In strain at break, PPTF was significantly weaker than CGF. Likewise, fibrin fiber thickness and crosslink density of PPTF were less than those of other membranes, and PPTF degraded faster than others. Although the centrifugal conditions are different, A-PRF and CGF are prepared by essentially identical mechanisms. Therefore, it is conceivable that both membranes have similar mechanical and chemical properties. Only PPTF, which was prepared by a different mechanism, was characterized as mechanically weaker and enzymatically more degradable.