Development of phytotoxicity and composition of a soil treated with olive mill wastewater (OMW): an incubation study
Development of phytotoxicity and composition of a soil treated with olive mill wastewater (OMW): an incubation study
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DOI：
10.1007/s11104-014-2241-3
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发表时间：
2014
期刊：
影响因子：
4.9
通讯作者：
C. Buchmann;A. Felten;B. Peikert;Katherine Muñoz;Nicole Bandow;Arnon Dag;Gabriele E. Schaumann
中科院分区：
文献类型：
--
作者：
C. Buchmann;A. Felten;B. Peikert;Katherine Muñoz;Nicole Bandow;Arnon Dag;Gabriele E. Schaumann
背景和目的在地中海国家产生的橄榄工厂废水（OMW）部分在土壤中处理。其潜在的命运机制和对植物生长的影响在很大程度上仍然未知。本研究旨在了解OMW有机质在土壤中的降解及其对植物的毒害作用。我们假设，OMW的植物毒性降低，其酚类组分的降解。MethodsIn 60天的培养研究，我们监测土壤呼吸，可提取的总酚含量（TPC）和碳同位素比值（δ 13 C）的OMW处理以色列土壤。采用加速溶剂提取（ASE）对土壤进行提取，并通过LC/MS对提取物中的四种酚类物质进行了示例性分析。采用独行菜种子萌发生物测定法测试了土壤和土壤提取物的植物毒性。结果OMW处理的土壤呼吸增加了2.5倍，具有两个呼吸最大值，表明添加的OMW-OM降解高达27%。确定了OMW-OM降解的四个阶段：（i）易降解OMW-OM的降解和酚类化合物的转化，（ii）植物毒性的中间抑制，（iii）植物毒性酚类化合物的降解和（iv）植物毒性化合物的显著物理固定化。最大限度地减少其物理固定和植物毒性效应。
Background and aimsOlive mill wastewater (OMW) generated in Mediterranean countries is partly disposed of on soil. Its underlying fate mechanisms and influences on plant growth are still largely unknown. Our goal was to understand OMW organic matter (OMW-OM) degradation in soil and its phytotoxic effects. We hypothesized that OMW phytotoxicity decreased with degradation of its phenolic components.MethodsIn a 60 day incubation study, we monitored soil respiration, extractable total phenolic content (TPC) and carbon isotope ratio (δ13C) of OMW treated Israeli soil. The soil was extracted using accelerated solvent extraction (ASE) and its extracts were exemplarily analyzed for four phenolic substances by LC/MS. Phytotoxicity of soil and soil extracts were tested using aLepidium sativumseed germination bioassay.ResultsSoil respiration was 2.5 times higher for OMW treated soil with two respiration maxima and indicated a degradation of up to 27 % of the added OMW-OM. Four phases of OMW-OM degradation were identified: (i) degradation of easily degradable OMW-OM and transformation of phenolic compounds, (ii) intermediate suppression of phytotoxicity, (iii) degradation of phytotoxic phenolic compounds and (iv) significant physical immobilization of phytotoxic compounds.ConclusionEnvironmental conditions during and after OMW disposal on soil ought to favor fast degradation of OMW-OM, minimizing their physical immobilization and phytotoxic effects.