Vasoactive intestinal peptide: a potent stimulator of adenosine 3':5'-cyclic monophosphate accumulation in gut carcinoma cell lines in culture.
Vasoactive intestinal peptide: a potent stimulator of adenosine 3':5'-cyclic monophosphate accumulation in gut carcinoma cell lines in culture.
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血管活性肠肽：培养的肠道癌细胞系中腺苷 3:5-环单磷酸积累的有效刺激剂。
DOI：
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发表时间：
1978
影响因子：
11.1
通讯作者：
G. Rosselin
中科院分区：
文献类型：
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作者：
M. Laburthe;M. Rousset;C. Boissard;G. Chevalier;A. Zweibaum;G. Rosselin
血管活性肠肽（VIP）是一种有效的刺激人结肠癌细胞系HT 29中腺苷3 '：5'-环磷酸（cAMP）积累的物质。cAMP积累对低至3 × 10（-12）M的VIP浓度敏感。在10（-9）M VIP中观察到VIP诱导的最大cAMP水平，约为基础水平的200倍。在3x 10（-10）M VIP下获得半最大cAMP产量。发现（125）I标记的VIP与HT 29细胞结合;这种结合被浓度在10（-10）和10（-7）M之间的未标记VIP竞争性抑制。使用2x 10（-9）M VIP时观察到结合的半数最大抑制。促胰液素也能促进HT 29细胞内cAMP的积累，但其作用强度仅为VIP的1/1000。在10（-7）M下测试的其他肽，如胰岛素、胰高血糖素、牛胰多肽、生长抑素、胆囊收缩素八肽、神经降压素和P物质，不刺激cAMP积累。前列腺素E（1）和儿茶酚胺刺激cAMP的产生，但效率分别为VIP的1/2.3和1/5.5。另一种来自肠道的恶性细胞系，人直肠肿瘤细胞系HRT 18，也对VIP敏感。在HRT 18细胞中，VIP刺激cAMP积累，在10（-8）M时达到最大效应;在约10（-9）M时观察到半最大刺激。这些结果表明，VIP受体的存在下，在两个恶性人肠细胞系（HT 29和HRT 18）在文化和研究VIP对细胞增殖的作用提供了一个模型。
Vasoactive intestinal peptide (VIP) is a potent and efficient stimulator of adenosine 3':5'-cyclic monophosphate (cAMP) accumulation in a human colon carcinoma cell line, HT 29. cAMP accumulation is sensitive to a concentration of VIP as low as 3x10(-12) M. Maximum VIP-induced cAMP levels were observed with 10(-9) M VIP and are about 200 times above the basal levels. Half-maximum cAMP production was obtained at 3x10(-10) M VIP. (125)I-Labeled VIP was found to bind to HT 29 cells; this binding was competitively inhibited by concentrations of unlabeled VIP between 10(-10) and 10(-7) M. Half-maximum inhibition of binding was observed with 2x10(-9) M VIP. Secretin also stimulated cAMP accumulation in HT 29 cells, but its effectiveness was 1/1000 that of VIP. The other peptides tested at 10(-7) M, such as insulin, glucagon, bovine pancreatic polypeptide, somatostatin, octapeptide of cholecystokinin, neurotensin, and substance P, did not stimulate cAMP accumulation. Prostaglandin E(1) and catecholamines stimulated cAMP production but were 1/2.3 and 1/5.5 as efficient as VIP, respectively. Another malignant cell line from the gut, the human rectal tumor cell line HRT 18, is also sensitive to VIP. In HRT 18 cells, VIP stimulated cAMP accumulation with a maximal effect at 10(-8) M; half-maximum stimulation was observed at about 10(-9) M. These results demonstrate the presence of VIP receptors in two malignant human intestinal cell lines (HT 29 and HRT 18) in culture and provide a model for studying the action of VIP on cell proliferation.