Intrinsic differences between the superficial and deep layers of the Xenopus ectoderm control primary neuronal differentiation
Intrinsic differences between the superficial and deep layers of the Xenopus ectoderm control primary neuronal differentiation
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DOI：
10.1016/s1534-5807(02)00113-2
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发表时间：
2002-02-01
影响因子：
11.8
通讯作者：
Papalopulu, N
中科院分区：
文献类型：
--
作者：
Chalmers, AD;Welchman, D;Papalopulu, N
在非洲爪哇，初级神经元在神经外胚层的深层分化得很早。相反，浅层的神经前体继续增殖。我们报道，浅层前体不同于深层前体，因为它们对bHLH基因的神经元促进活性、显性负X-Delta-1、成纤维细胞生长因子-8或来自组织者的信号不敏感。在这个系统中，神经元的分化是由早期建立的、内在的、细胞自主的前体细胞分化能力的差异来指导的。这种差异可能部分由ESR6e基因控制，ESR6e基因是分裂增强因子家族的一个基因，它在胚泡晚期的浅层表达，当异位表达时，抑制深层的初级神经发生。
In Xenopus, primary neurons differentiate early, in the deep layer of the neuroectoderm. In contrast, the neural precursors of the superficial layer continue to proliferate. We report that superficial layer precursors differ from deep layer precursors in that they are refractory to the neuronal-promoting activity of bHLH genes, dominant-negative X-Delta-1, FGF-8, or signals from the organizer. In this system, neuronal differentiation is guided by an early established, intrinsic, cell-autonomous difference in the competence of the precursor cells to differentiate. This difference may be controlled in part by ESR6e, a bHLH gene of the Enhancer-of-split family, which is expressed in the superficial layer of the late blastula and when expressed ectopically suppresses primary neurogenesis in the deep layer.