Oscillatory Ca2+ Signals Evoked by Cholinergic Stimulation in Isolated Rat Pancreatic Duct Cells
Oscillatory Ca2+ Signals Evoked by Cholinergic Stimulation in Isolated Rat Pancreatic Duct Cells
复制标题
胆碱能刺激离体大鼠胰管细胞引起的振荡 Ca2 信号
DOI：
10.2220/biomedres.21.283
复制
发表时间：
2000
影响因子：
1.2
通讯作者：
Y. Habara
中科院分区：
文献类型：
--
作者：
H. Takahashi;M. J. Kim;K. Abe;Y. Habara
已知乙酰胆碱可增强胰管系统分泌素刺激的碳酸氢盐分泌。最近的研究表明，这种现象至少部分归因于激动剂诱导的胰管细胞内Ca ~（2+）浓度（[Ca ~（2+）] i）升高。为了定义这些[Ca 2 +]、信号的时空动态，从大鼠胰腺中分离出小叶内导管和小叶间导管的短片段，并使用钙敏感荧光探针Fura-2通过数字图像显微镜进行检查。雅阁与以前的报告一致，用卡巴胆碱刺激的导管节段一致地显示[Ca 2 +]，升高，阿托品特异性抑制。目前的图像分析还表明，在刺激过程中，导管上皮细胞中的[Ca 2 +]，水平的细微波动不均匀，这表明胰管细胞的响应的异步振荡模式。胰管系统实验乙酰胆碱
Acetylcholine has been known to potentiate secretin-stimulated bicarbonate secretion by the pancreatic duct system. Recent studies have shown that this phenomenon is at least partially attributable to the agonist-induced elevation ofintracellular Ca” concentration ([Ca2+],) in pancreatic duct cells. In order to define spatiotemporal dynamics of these [Ca2+], signals, short segments of intralobular and interlobular ducts were isolated from the rat pancreas and examined by digital image microscopy using a calcium-sensitive, fluorescent probe, Fura-2. In accord with previous reports by microspectrofluorometry, duct segments stimulated with carbachol consistently displayed [Ca2+], rises, which were specifically suppressed by atropine. Present image analyses additionally demonstrated a fine fluctuate unevenness of the [Ca2+], level in the duct epithelium during the stimulation, suggesting an asynchronous oscillatory mode of response of the pancreatic duct cells. The pancreatic duct system Experiments acetylcholine the