NUCLEOTIDE-SEQUENCE AND SECONDARY STRUCTURE OF APPLE SCAR SKIN VIROID
NUCLEOTIDE-SEQUENCE AND SECONDARY STRUCTURE OF APPLE SCAR SKIN VIROID
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DOI：
10.1093/nar/15.17.7045
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发表时间：
1987-09-11
影响因子：
14.9
通讯作者：
KOGANEZAWA, H
中科院分区：
文献类型：
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作者：
HASHIMOTO, J;KOGANEZAWA, H
测定了苹果疤皮类病毒（ASSV）的核苷酸全序列，并提出了可能的二级结构。单链环状ASSV RNA由330个核苷酸组成，可以呈现杆状构象，具有所有已知类病毒的广泛碱基配对特征。ASSV与其他类病毒的同源性较低，缺乏中央保守区。这表明ASSV应该被分配到一个单独的类病毒组。然而，同源序列与马铃薯纺锤块茎类病毒（PSTV）在ASSV发生在有限的和分散的区域的两个类病毒。这些同源区域落入先前已由Keese和Symons（Proc.Natl. Acad. Sci. USA. 82，4582-4586，1985）。
The complete nucleotide sequence of apple scar skin viroid(ASSV) has been established, and a probable secondary structure is proposed. A single-stranded circular ASSV RNA consists of 330 nucleotides and can assume the rodlike conformation with extensive base-pairing characteristic of all the known viroids. ASSV shows low sequence homologies with other viroids and lacks the central conserved region. These indicate that ASSV should be allocated to a separate viroid group. However, homologous sequences with potato spindle tuber viroid(PSTV) in ASSV occur in limited and scattered regions of both viroids. These homologous regions fall within the particular domains in the viroid domain model which has been previously proposed by Keese and Symons(Proc. Natl. Acad. Sci. USA. 82, 4582–4586, 1985).