AtDOF5.4/OBP4, a DOF Transcription Factor Gene that Negatively Regulates Cell Cycle Progression and Cell Expansion in Arabidopsis thaliana.
AtDOF5.4/OBP4, a DOF Transcription Factor Gene that Negatively Regulates Cell Cycle Progression and Cell Expansion in Arabidopsis thaliana.
复制标题
AtDOF5.4/OBP4，一种负向调节拟南芥细胞周期进展和细胞扩增的 DOF 转录因子基因
DOI：
10.1038/srep27705
复制
发表时间：
2016-06-14
影响因子：
4.6
通讯作者：
Cai W
中科院分区：
文献类型：
--
作者：
Xu P;Chen H;Ying L;Cai W
与动物不同，植物的发育涉及连续的器官形成，这需要严格的细胞增殖调控。核心细胞周期机制在植物和动物中是保守的，但植物已经开发出新的机制，可以精确地调节细胞增殖以应对内部和外部刺激。在这里，我们报告DOF转录因子OBP 4负调控细胞增殖和扩增。OBP 4是一种核蛋白。OBP 4的组成型和诱导型过表达减少了细胞的大小和数量，导致植株矮化。诱导过表达的OBP 4在拟南芥中也促进了早期内循环的启动并抑制了细胞的扩增，而诱导过表达的OBP 4融合到VP 16激活结构域的拟南芥中延迟了内循环的启动并促进了植物的生长。此外，基因表达分析表明，细胞周期调节因子和细胞壁扩张因子在OBP 4过表达系中大幅下调。结合体内ChIP分析的短期诱导分析表明，OBP 4靶向CyclinB 1;1，CDKB 1; 1和XTH基因。这些结果有力地表明，OBP 4是细胞周期进程和细胞生长的负调控因子。这些发现增加了我们对植物细胞周期转录调控的理解。
In contrast to animals, plant development involves continuous organ formation, which requires strict regulation of cell proliferation. The core cell cycle machinery is conserved across plants and animals, but plants have developed new mechanisms that precisely regulate cell proliferation in response to internal and external stimuli. Here, we report that the DOF transcription factorOBP4negatively regulates cell proliferation and expansion. OBP4 is a nuclear protein. Constitutive and inducible overexpression ofOBP4reduced the cell size and number, resulting in dwarf plants. Inducible overexpression ofOBP4inArabidopsisalso promoted early endocycle onset and inhibited cell expansion, while inducible overexpression ofOBP4fused to the VP16 activation domain inArabidopsisdelayed endocycle onset and promoted plant growth. Furthermore, gene expression analysis showed that cell cycle regulators and cell wall expansion factors were largely down-regulated in theOBP4overexpression lines. Short-term inducible analysis coupled within vivoChIP assays indicated thatOBP4targets theCyclinB1;1,CDKB1;1andXTHgenes. These results strongly suggest thatOBP4is a negative regulator of cell cycle progression and cell growth. These findings increase our understanding of the transcriptional regulation of the cell cycle in plants.