In Vivo PET Tracking of 89Zr-Labeled Vγ9Vδ2 T Cells to Mouse Xenograft Breast Tumors Activated with Liposomal Alendronate
In Vivo PET Tracking of 89Zr-Labeled Vγ9Vδ2 T Cells to Mouse Xenograft Breast Tumors Activated with Liposomal Alendronate
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DOI：
10.1016/j.ymthe.2018.10.006
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发表时间：
2019-01-02
影响因子：
12.4
通讯作者：
de Rosales, Rafael T. M.
中科院分区：
文献类型：
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作者：
Man, Francis;Lim, Lindsay;de Rosales, Rafael T. M.
Gammadelta T(Gamma Delta-T)细胞因其细胞毒性、易扩增和良好的安全性而成为肿瘤学过继免疫治疗的有力候选者。伽马增量T细胞疗法的发展将受益于非侵入性细胞跟踪方法和更多针对肿瘤部位的靶向性。本文报道了用正电子发射断层扫描(PET)技术对活体内V-γ9V-2T细胞进行示踪的研究。在体外，我们显示了标记的V-伽马9V Delta 2T细胞保持了它们的活力、增殖能力和抗癌细胞毒性，同时对DNA的损伤最小。
Gammadelta T (gamma delta-T) cells are strong candidates for adoptive immunotherapy in oncology due to their cytotoxicity, ease of expansion, and favorable safety profile. The development of gamma delta-T cell therapies would benefit from non-invasive celltracking methods and increased targeting to tumor sites. Here we report the use of [Zr-89] Zr(oxinate) 4 to track V gamma 9V delta 2 T cells in vivo by positron emission tomography (PET). In vitro, we showed that Zr-89-labeled V gamma 9V delta 2T cells retained their viability, proliferative capacity, and anti-cancer cytotoxicity with minimal DNA damage for amounts of Zr-89