Factors Promoting Neurite Outgrowth During Deer Antler Regeneration
Factors Promoting Neurite Outgrowth During Deer Antler Regeneration
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DOI：
10.1002/jnr.22459
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发表时间：
2010-11-01
影响因子：
4.2
通讯作者：
Nieto-Diaz, Manuel
中科院分区：
文献类型：
--
作者：
Pita-Thomas, Wolfgang;Nieto-Sampedro, Manuel;Nieto-Diaz, Manuel
每年，公鹿的鹿角都会完全再生。在这个过程中，鹿角被感觉纤维重新支配，以任何成年哺乳动物记录的最高速度生长。尽管它具有临床潜力，但只有少数研究涉及这种迷人的现象。在可能的因素之一的快速增长的鹿茸的神经支配，鹿茸的内分泌和旁分泌因素的影响进行了评估，使用在体外测定感觉神经突起的生长。我们发现，鹿茸分泌的可溶性分子，即覆盖鹿茸的改良皮肤，强烈促进神经突的生长。使用特异性阻断抗体，我们证明神经生长因子是这些效应的部分原因，尽管其他未鉴定的分子也参与其中。相反，内分泌血清因子和鹿茸基质都不促进神经突的生长，尽管鹿茸基质来自深鹿茸层导致神经突的生长方向。两者合计，我们的研究结果指出，在深天鹅绒的环境，促进定向轴突生长，在鹿茸神经支配的分布一致的存在。(c)2010 Wiley-Liss，Inc.
Every year male deers completely regenerate their antlers. During this process, antlers are reinnervated by sensory fibers, growing at the highest rate recorded for any adult mammal. Despite its clinical potential, only a few studies have dealt with this fascinating phenomenon. Among the possible factors underlying fast growth of the antler's innervation, the effects of the antler's endocrine and paracrine factors were evaluated, using an in vitro assay for sensory neurite growth. We found that soluble molecules secreted by the velvet, the modified skin that covers the antler, strongly promote neurite outgrowth. Using specific blocking antibodies, we demonstrated that nerve growth factor is partially responsible for these effects, although other unidentified molecules are also involved. On the contrary, neither endocrine serum factors nor antler substrates promoted neurite outgrowth, although antler substrata from deep velvet layers cause neurite outgrowth orientation. Taken together, our results point to the existence in the deep velvet of an environment that promotes oriented axon growth, in agreement with the distribution of the antler innervation. (c) 2010 Wiley-Liss, Inc.