Prior stimulation of antigen-presenting cells with Lactobacillus regulates excessive antigen-specific cytokine responses in vitro when compared with Bacteroides
Prior stimulation of antigen-presenting cells with Lactobacillus regulates excessive antigen-specific cytokine responses in vitro when compared with Bacteroides
复制标题
与拟杆菌相比，用乳杆菌预先刺激抗原呈递细胞可在体外调节过度的抗原特异性细胞因子反应
DOI：
--
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
S. Kaminogawa
中科院分区：
文献类型：
--
作者：
Masato Tsuda;A. Hosono;Tsutomu Yanagibashi;S. Hachimura;K. Hirayama;K. Itoh;Kyoko Takahashi;S. Kaminogawa
过敏的发展与肠道微生物群的差异有关。因此，这表明，不同属的细菌诱导的免疫应答可能通过适应性免疫和先天免疫来调节。在这项研究中，我们研究了抗原特异性免疫反应是否受到体外不同属肠道细菌刺激的影响。用卵清蛋白（OVA）和肠道细菌刺激从无菌卵清蛋白（OVA）特异性T细胞受体转基因（OVA-Tg）小鼠分离的肠系膜淋巴结（MLN）细胞。常规小鼠而非无菌小鼠的盲肠内容物可诱导OVA特异性细胞因子的产生。在小鼠肠道细菌中，产酸拟杆菌（BA）增强OVA特异性IFN-γ和IL-10的产生，而约氏乳杆菌（LA）仅增加OVA特异性IL-10的产生。分析了无菌Balb/c小鼠抗原呈递细胞（APC）的细胞表面分子表达和细胞因子产生。与LA相比，BA增加了APCs上MHCII和共刺激分子的表达。BA可增加IL-6和IL-10的产生，但诱导的IL-12 p40低于LA。为了检查由肠道细菌预先刺激APC对诱导抗原特异性免疫应答的影响，在与OVA、来自OVA-Tg小鼠的CD 4 + T细胞和用细菌或未用细菌预刺激的APC共培养后测定细胞因子产生。用LA预刺激的APC不增强OVA特异性细胞因子的产生，而BA刺激OVA特异性IL-10的产生。这些结果表明，先前的刺激肠道免疫细胞的乳酸杆菌可能会调节过度的抗原特异性细胞因子反应，通过APC相比，先前的刺激类杆菌。
The development of allergy is related to differences in the intestinal microbiota. Therefore, it is suggested that the immune responses induced by different genera of bacteria might be regulated through adaptive as well as innate immunity. In this study, we examined whether antigen-specific immune responses were affected by stimulation with the different genera of intestinal bacteria in vitro. Mesenteric lymph node (MLN) cells isolated from germ-free ovalbumin (OVA)-specific T cell receptor transgenic (OVA-Tg) mice were stimulated with OVA and intestinal bacteria. Cecal contents from conventional mice but not germ-free mice could induce OVA-specific cytokine production. Among the murine intestinal bacteria, Bacteroides acidofaciens (BA) enhanced OVA-specific IFN-γ and IL-10 production while Lactobacillusjohnsonii (LA) increased OVA-specific IL-10 production only. The expression of cell surface molecules and cytokine production by antigen-presenting cells (APCs) from germ-free Balb/c mice were analyzed. BA increased the expression of MHC II and co-stimulatory molecules on APCs compared with LA. BA increased IL-6 and IL-10 production but induced less IL-12p40 than LA. To examine the effects of prior stimulation of APCs by intestinal bacteria on the induction of antigen-specific immune responses, cytokine production was determined following co-culture with OVA, CD4+ T cells from OVA-Tg mice, and APCs which were pre-stimulated with the bacteria or not. APCs pre-stimulated with LA did not enhance OVA-specific cytokine production while BA stimulated OVA-specific IL-10 production. These results suggest that the prior stimulation of intestinal immunocytes by Lactobacillus might regulate excessive antigen-specific cytokine responses via APCs when compared with prior stimulation by Bacteroides.