Co-precipitation of DEAE-dextran coated SPIONs: how synthesis conditions affect particle properties, stem cell labelling and MR contrast
Co-precipitation of DEAE-dextran coated SPIONs: how synthesis conditions affect particle properties, stem cell labelling and MR contrast
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DOI：
10.1002/cmmi.1700
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发表时间：
2016-09-01
影响因子：
--
通讯作者：
Adams, Dave J.
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文献类型：
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作者：
Barrow, Michael;Taylor, Arthur;Adams, Dave J.
超顺磁性氧化铁纳米颗粒（SPION）广泛用作磁共振成像（MRI）追踪干细胞的造影剂。可内化到细胞中而不改变细胞活力或表型的氧化铁总质量是生成对比的重要标准，SPION 专为有效标记干细胞而设计，可提高检测灵敏度。尽管已知共沉淀反应中聚合物和铁盐比例的变化会影响 SPION 的理化性质，特别是核心尺寸，但这些合成条件对干细胞标记和磁共振 (MR) 对比度的影响尚未确定。在这里，我们合成了一系列具有非常相似的流体动力学直径和表面电荷但聚合物含量不同的阳离子 SPION。我们研究了共沉淀反应中聚合物的量如何影响核心尺寸，并不仅调节 SPION 的磁性，还调节它们对干细胞的吸收。具有最大核心尺寸和最低聚合物含量的 SPION 表现出最高的磁化强度和弛豫率。这些颗粒还具有最高的吸收效率，并且不会对干细胞的活力或功能产生任何有害影响。然而，对于细胞内内化的所有颗粒，T-2 和 T-2* 弛豫率与 SPION 的核心尺寸无关。我们的结果表明，细胞吸收的铁的相对质量是 MR 造影剂生成的主要决定因素，并表明 SPION 吸收的程度可以通过共沉淀反应中使用的聚合物的量来调节。版权所有 (C) 2016 约翰·威利父子有限公司
Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used as contrast agents for stem cell tracking using magnetic resonance imaging (MRI). The total mass of iron oxide that can be internalised into cells without altering their viability or phenotype is an important criterion for the generation of contrast, with SPIONs designed for efficient labelling of stem cells allowing for an increased sensitivity of detection. Although changes in the ratio of polymer and iron salts in co-precipitation reactions are known to affect the physicochemical properties of SPIONs, particularly core size, the effects of these synthesis conditions on stem cell labelling and magnetic resonance (MR) contrast have not been established. Here, we synthesised a series of cationic SPIONs with very similar hydrodynamic diameters and surface charges, but different polymer content. We have investigated how the amount of polymer in the co-precipitation reaction affects core size and modulates not only the magnetic properties of the SPIONs but also their uptake into stem cells. SPIONs with the largest core size and lowest polymer content presented the highest magnetisation and relaxivity. These particles also had the greatest uptake efficiency without any deleterious effect on either the viability or function of the stem cells. However, for all particles internalised in cells, the T-2 and T-2* relaxivity was independent of the SPION's core size. Our results indicate that the relative mass of iron taken up by cells is the major determinant of MR contrast generation and suggest that the extent of SPION uptake can be regulated by the amount of polymer used in co-precipitation reactions. Copyright (C) 2016 John Wiley & Sons, Ltd.