New MHC class Ia domain lineages in rainbow trout (Oncorhynchus mykiss) which are shared with other fish species
New MHC class Ia domain lineages in rainbow trout (Oncorhynchus mykiss) which are shared with other fish species
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DOI：
10.1016/j.fsi.2004.07.007
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发表时间：
2005-03-01
影响因子：
4.7
通讯作者：
Ototake, M
中科院分区：
文献类型：
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作者：
Kiryu, I;Dijkstra, JM;Ototake, M
鲑科鱼类的主要组织相容性复合体(MHC)la类基因由一个基因座编码，可能是迄今所描述的最高的等位基因多样性。非常不同的区域谱系的各种组合有助于等位基因的多样性。建立了一个广泛的聚合酶链式反应调查，区分大多数区域谱系及其组合。这一调查对研究鲑鱼MHC la类变异具有实用价值。在目前的研究中，它被用来寻找新的结构域谱系。这项调查应用于日本的24个孵化场品系，确定了两个新的虹鲑α1谱系和一个新的虹鲑α2谱系。在大西洋鲑鱼中已经描述了Alpha2血统和其中一个Alpha1血统，但另一个Alpha1血统是新的。新发现的鲑鱼Alpha1谱系在进化上非常古老。这项研究应该是迄今为止对非常深的MHC la类谱系的最广泛的描述：六个鲑鱼α1谱系与非鲑鱼序列聚集在一起，而之前的研究只提到了两个鲑鱼α1谱系。尽管外显子改组事件对鲑鱼MHC la类变异有显著贡献，但对800条鲑鱼兄弟姐妹的分析并未在单一世代内检测到此类事件。(C)2004爱思唯尔有限公司。保留所有权利。
Major histocompatibility complex (MHC) class la genes in salmonid fishes are encoded by a single locus with probably the highest allelic diversity ever described. Various combinations of very different domain lineages contribute to the diversity of alleles. An extensive PCR survey distinguishing most domain lineages and their combinations was established. This survey has practical value for researchers investigating salmonid MHC class la variation. In the present study it was used to find new domain lineages. Applied for 24 hatchery strains in Japan, the survey identified two new rainbow trout alpha1 lineages and one new rainbow trout alpha2 lineage. The alpha2 lineage and one of the alpha1 lineages had been described in Atlantic salmon, but the other alpha1 lineage is novel. The newly identified trout alpha1 lineages are evolutionary very old. The present study should be the most extensive description of very deep MHC class la lineages to date: six trout alpha1 lineages cluster with non-salmonid sequences whereas previous studies mentioned this for only two salmonid alpha1 lineages. Although exon-shuffling events significantly contributed to salmonid MHC class la variation, analysis of 800 trout siblings did not detect such events within a single generation. (C) 2004 Elsevier Ltd. All rights reserved.