The Aquaporin TaPIP2;10 Confers Resistance to Two Fungal Diseases in Wheat
The Aquaporin TaPIP2;10 Confers Resistance to Two Fungal Diseases in Wheat
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水通道蛋白 TaPIP2;10 赋予小麦对两种真菌病害的抵抗力
DOI：
10.1094/phyto-02-21-0048-r
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发表时间：
2021
期刊：
影响因子：
3.2
通讯作者：
Hansong Dong
中科院分区：
文献类型：
--
作者：
Xiaobing Wang;Kai Lu;Xiaohui Yao;Fubin Wang;Degong Wu;Jinfeng Peng;Xiaochen Chen;Junli Du;Jingyu Ma;Lei Chen;Shenshen Zou;Chunling Zhang;Meixiang Zhang;Hansong Dong
植物利用质膜内在蛋白（PIP）家族的水通道蛋白（AQP）输入环境底物，从而影响各种过程，例如由信号分子过氧化氢（H2 O2）调节的细胞反应。普通小麦（Triticum aestivum）含有PIP家族的24个候选成员，命名为TaPIP 1;1至TaPIP 1;12和TaPIP 2;1至TaPIP 2;12。这些TaPIP候选植物均未对其来源植物的底物选择性或防御反应进行表征。在这里，我们报告说，T。aestivumAQP TaPIP 2; 10促进H2 O2的细胞吸收，赋予对白粉病和镰刀菌头枯病的抗性，这两种毁灭性的真菌病害在世界各地的小麦。在小麦中，质外体H2 O2信号是由真菌攻击诱导的，而TaPIP 2;10被刺激将H2 O2转运到细胞质中，在那里它激活防御反应以限制进一步的攻击。TaPIP 2;10介导的H2 O2转运在病原相关分子模式触发的植物免疫（PTI）中是必不可少的。典型的PTI反应是由真菌感染诱导的，并通过TaPIP 2; 10基因的过表达而增强。TaPIP 2; 10基因的过表达导致小麦对白粉病的抗性增强70%，对赤霉病的抗性增强86%。TaPIP 2; 10过表达降低了病害的严重程度，使小麦籽粒产量增加了37%以上。这些结果验证了使用免疫相关的AQP同时提高作物产量和免疫力的可行性。
Plants employ aquaporins (AQPs) of the plasma membrane intrinsic protein (PIP) family to import environmental substrates, thereby affecting various processes, such as the cellular responses regulated by the signaling molecule hydrogen peroxide (H2O2). Common wheat (Triticum aestivum) contains 24 candidate members of the PIP family, designated as TaPIP1;1 to TaPIP1;12 and TaPIP2;1 to TaPIP2;12. None of these TaPIP candidates have been characterized for substrate selectivity or defense responses in their source plant. Here, we report thatT. aestivumAQP TaPIP2;10 facilitates the cellular uptake of H2O2to confer resistance against powdery mildew and Fusarium head blight, two devastating fungal diseases in wheat throughout the world. In wheat, the apoplastic H2O2signal is induced by fungal attack, while TaPIP2;10 is stimulated to translocate this H2O2into the cytoplasm, where it activates defense responses to restrict further attack. TaPIP2;10-mediated transport of H2O2is essential for pathogen-associated molecular pattern-triggered plant immunity (PTI). Typical PTI responses are induced by the fungal infection and intensified by overexpression of theTaPIP2;10gene.TaPIP2;10overexpression causes a 70% enhancement in wheat resistance to powdery mildew and an 86% enhancement in resistance to Fusarium head blight. By reducing the disease severities,TaPIP2;10overexpression brings about >37% increase in wheat grain yield. These results verify the feasibility of using an immunity-relevant AQP to concomitantly improve crop productivity and immunity.