Signal-anchor sequences are an essential factor for the Golgi-plasma membrane localization of type II membrane proteins
Signal-anchor sequences are an essential factor for the Golgi-plasma membrane localization of type II membrane proteins
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信号锚序列是 II 型膜蛋白在高尔基体膜上定位的重要因素
DOI：
10.1080/09168451.2018.1484272
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Mukai Yuri
中科院分区：
文献类型：
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作者：
Kikegawa Tatsuki;Yamaguchi Takuya;Nambu Ryohei;Etchuya Kenji;Ikeda Masami;Mukai Yuri
尽管膜蛋白的细胞内定位的机制的研究，每个膜蛋白定位到内质网，高尔基体，和质膜的分泌途径的具体机制尚不清楚。在这项研究中，判别分析的内质网，高尔基体和质膜定位的II型膜蛋白进行了使用位置特异性评分矩阵来自周围的信号锚序列的氨基酸倾向。评估了信号锚周围的序列是识别每个定位组的因素的可能性。高尔基体和质膜定位的II型膜蛋白之间的歧视精度高达90%，这表明，除了其他因素，信号锚周围的序列是一个重要组成部分的选择机制的高尔基体和质膜定位。这些结果可以改善膜蛋白用于药物递送和治疗应用的用途。
Despite studies of the mechanism underlying the intracellular localization of membrane proteins, the specific mechanisms by which each membrane protein localizes to the endoplasmic reticulum, Golgi apparatus, and plasma membrane in the secretory pathway are unclear. In this study, a discriminant analysis of endoplasmic reticulum, Golgi apparatus and plasma membrane-localized type II membrane proteins was performed using a position-specific scoring matrix derived from the amino acid propensity of the sequences around signal-anchors. The possibility that the sequence around the signal-anchor is a factor for identifying each localization group was evaluated. The discrimination accuracy between the Golgi apparatus and plasma membrane-localized type II membrane proteins was as high as 90%, indicating that, in addition to other factors, the sequence around signal-anchor is an essential component of the selection mechanism for the Golgi and plasma membrane localization. These results may improve the use of membrane proteins for drug delivery and therapeutic applications.