Aptamer selection express: a novel method for rapid single-step selection and sensing of aptamers.
Aptamer selection express: a novel method for rapid single-step selection and sensing of aptamers.
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发表时间：
2008-12
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通讯作者：
Maomian Fan;Shelly Roper McBurnett;C. Andrews;A. Allman;J. Bruno;J. Kiel
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作者：
Maomian Fan;Shelly Roper McBurnett;C. Andrews;A. Allman;J. Bruno;J. Kiel
在这里，我们描述了一种新的DNA捕获元件(DCE)传感系统，基于双链适配子的猝灭和去猝灭。该系统具有很好的灵敏度和热稳定性。在猝灭、去猝灭和分离由不同适配子(均由SELEX选择)制成的DCE系统时，开发了一种快速选择适配子的替代方法-适配子选择快速(ASExp)。这一方法已被用于针对不同类型的靶(炭疽芽胞杆菌孢子、苏云金芽胞杆菌孢子、MS-2噬菌体、卵清蛋白和肉毒神经毒素)选择适配子。对ASExp选择的肉毒神经毒素适配子制成的DCE系统进行了研究。研究结果表明，ASExp可用于DCE传感系统中适体的快速筛选。
Here we describe a new DNA capture element (DCE) sensing system, based on the quenching and dequenching of a double-stranded aptamer. This system shows very good sensitivity and thermal stability. While quenching, dequenching, and separating the DCE systems made from different aptamers (all selected by SELEX), an alternative method to rapidly select aptamers was developed-the Aptamer Selection Express (ASExp). This process has been used to select aptamers against different types of targets (Bacillus anthracis spores, Bacillus thuringiensis spores, MS-2 bacteriophage, ovalbumin, and botulinum neurotoxin). The DCE systems made from botulinum neurotoxin aptamers selected by ASExp have been investigated. The results of this investigation indicate that ASExp can be used to rapidly select aptamers for the DCE sensing system.