Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae
Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae
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DOI：
10.1111/j.1365-2958.2005.04917.x
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发表时间：
2005-12-01
影响因子：
3.6
通讯作者：
Fassler, JS
中科院分区：
文献类型：
--
作者：
He, XJ;Fassler, JS
酿酒酵母Yap1p和Skn7p转录因子协同激活氧化应激反应（OSR）基因。虽然在一些OSR基因中已经鉴定出Yap1p和Skn7p氧化应激反应元件（YRE， OSRE），但许多OSR基因缺乏这些元件。在本研究中，我们以复杂的、氧化反应性的CCP1启动子作为模型来研究氧化应激激活的顺式作用元件。除了共识的YRE和OSRE序列外，在CCP1启动子中还发现了新的Yap1p和Skn7p结合位点。这些新位点被发现介导依赖于Yap1p和Skn7p的OSR基因的激活，包括以前认为缺乏Yap1p和Skn7p结合位点的TSA1和CTT1。在一组179个OSR基因的启动子区域发现了新的YREs和OSREs。新的Yap1p和Skn7p结合位点的广泛存在强烈表明，Yap1p和Skn7p的直接结合激活了比以前认为的更多的OSR基因。
The Saccharomyces cerevisiae Yap1p and Skn7p transcription factors collaborate in the activation of oxidative stress response (OSR) genes. Although Yap1p and Skn7p oxidative stress response elements (YRE, OSRE) have been characterized and identified in some OSR genes, many OSR genes lack such elements. In this study, the complex, oxidative responsive, CCP1 promoter was used as a model to investigate the cis-acting elements responsible for activation by oxidative stress. In addition to consensus YRE and OSRE sequences, novel Yap1p and Skn7p binding sites were identified in the CCP1 promoter. These new sites were found to mediate Yap1p- and Skn7p-dependent activation of OSR genes including TSA1 and CTT1 previously thought to lack Yap1p and Skn7p binding sites. The novel YREs and OSREs were found to be enriched in the promoter regions of a set of 179 OSR genes. The widespread existence of novel Yap1p and Skn7p binding sites strongly suggest that direct binding of Yap1p and Skn7p is responsible for activation of many more OSR genes than previously believed.