Reducing Physical Adsorption of Enzymes by Surface Modification of Carbon Black for High-Current-Density Biofuel Cells
Reducing Physical Adsorption of Enzymes by Surface Modification of Carbon Black for High-Current-Density Biofuel Cells
复制标题
通过炭黑表面改性减少高电流密度生物燃料电池中酶的物理吸附
DOI：
10.1149/2.0181413jes
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发表时间：
2014
影响因子：
3.9
通讯作者：
Yuhei Oshiba ,and Takeo Yamaguchi
中科院分区：
文献类型：
--
作者：
Takanori Tamaki;Tomoharu Sugiyama;Masahiro Mizoe;Yuhei Oshiba ,and Takeo Yamaguchi
减少酶在炭黑上的物理吸附和由此产生的失活是提高酶生物燃料电池电流密度从而使其在广泛应用中使用的关键因素。研究了炭黑表面处理对葡萄糖氧化酶(GOx)物理吸附的影响。然后用一种非物理吸附的方法将GOX固定在高比表面积的三维碳电极上。酸处理后的阴离子聚合物接枝聚合和炭黑与聚乙二醇(PEG)的混合都有效地减少了Gox的物理吸附。聚乙二烯表面修饰和硫酸铵沉淀法固定化GOX与交联剂的结合，提高了聚乙烯基二茂铁-丙烯酰胺接枝炭黑电极的葡萄糖氧化电流密度。
Reducing the physical adsorption of enzymes on carbon black and their resultant deactivation are the key factors to increasing the current density of enzymatic biofuel cells, and thus enabling their use in a wide range of applications. The effect of the surface treatment of carbon black on the physical adsorption of glucose oxidase (GOx) was investigated. GOx was then immobilized on a high-surface-area three-dimensional carbon electrode using a method other than physical adsorption. Acid treatment followed by graft polymerization of an anionic polymer and the mixing of carbon black with polyethylene glycol (PEG) were both effective in reducing the physical adsorption of GOx. The combination of the surface modification using PEG and the immobilization of GOx via ammonium sulfate precipitation with crosslinking increased the glucose-oxidation current density obtained in an electrode fabricated using poly (vinylferrocene-co-acrylamide)-grafted carbon black.