Recognition Properties of Processing α‐Glucosidase I and α‐Glucosidase II
Recognition Properties of Processing α‐Glucosidase I and α‐Glucosidase II
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α-葡萄糖苷酶 I 和 α-葡萄糖苷酶 II 加工的识别特性
DOI：
10.1081/car-120030022
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发表时间：
2004
影响因子：
1
通讯作者：
A. Takatsuki
中科院分区：
文献类型：
--
作者：
W. Hakamata;M. Muroi;T. Nishio;T. Oku;A. Takatsuki
摘要制备了4种可能的对硝基苯基α-D-吡喃葡萄糖苷（PNP Glc）单脱氧衍生物和1-氨基-2，6-脱水-1-脱氧-D-甘油-D-碘-庚糖醇衍生物，并将其用作大鼠肝脏加工α-葡萄糖苷酶的底物和抑制剂。α-葡萄糖苷酶II水解PNP Glc的2-脱氧衍生物（1）; 1的水解比PNP Glc的水解更快。这些结果表明，C-2羟基的存在对于α-葡萄糖苷酶II的作用不是必需的。相反，PNP Glc和PNP Glc 1-4的所有脱氧衍生物抑制α-葡糖苷酶I。这些结果表明，α-葡糖苷酶I不一定需要糖基部分的所有羟基与酶结合。2，6-脱水-1-苯甲酰胺-D-甘油基-D-氨基-庚糖醇（11），末端为苯基，可抑制α-葡萄糖苷酶I和α-葡萄糖苷酶II。α-葡萄糖苷酶I和II均显示出相同的糖苷配基特异性。当测定探针5-12在细胞水平抑制α-葡糖苷酶加工的能力时，未观察到对糖蛋白加工的影响。
Abstract All four possible monodeoxy derivatives of p‐nitrophenyl α‐D‐glucopyranoside (PNP Glc) and 1‐amino‐2,6‐anhydro‐1‐deoxy‐D‐glycero‐D‐ido‐heptitol derivatives were prepared and used as substrates and inhibitors of rat liver processing α‐glucosidases. α‐Glucosidase II hydrolyzed the 2‐deoxy derivative of PNP Glc (1); the hydrolysis of 1 was more rapid than that of PNP Glc. These results indicate that the presence of a C‐2 hydroxyl group is not essential for the action of α‐glucosidase II. In contrast, PNP Glc and all of the deoxy derivatives of PNP Glc 1–4 inhibited α‐glucosidase I. These results indicate that α‐glucosidase I does not necessarily need all of the hydroxyl groups of the glycon moiety for binding to the enzyme. 2,6‐Anhydro‐1‐benzamide‐D‐glycero‐D‐ido‐heptitol (11), with a terminal phenyl group, inhibited α‐glucosidase I and α‐glucosidase II. Both α‐glucosidase I and II showed the same aglycon specificities. When probes 5–12 were assayed for their ability to inhibit processing by α‐glucosidases at the cellular level, no effects on glycoprotein processing were observed.
DOI：
10.1016/s0021-9258(18)33975-9
发表时间：
1982-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Douglas;Burns;O. Touster
通讯作者：
Douglas;Burns;O. Touster
参与牛乳腺 N 连接糖蛋白加工的葡萄糖苷酶 I 的纯化和表征。
DOI：
10.1042/bj2470555
发表时间：
1987
期刊：
The Biochemical journal
影响因子：
--
作者：
Shailubhai,K;Pratta,MA;Vijay,IK
通讯作者：
Vijay,IK