Viscoelastic Behavior of Embroidered Scaffolds for ACL Tissue Engineering Made of PLA and P(LA-CL) After In Vitro Degradation
Viscoelastic Behavior of Embroidered Scaffolds for ACL Tissue Engineering Made of PLA and P(LA-CL) After In Vitro Degradation
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DOI：
10.3390/ijms20184655
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发表时间：
2019-09-02
影响因子：
5.6
通讯作者：
Breier, Annette
中科院分区：
文献类型：
--
作者：
Hahn, Judith;Schulze-Tanzil, Gundula;Breier, Annette
前交叉韧带（ACL）断裂是最常见的膝关节韧带损伤。目前应用的重建方法在移植物可用性和机械性能方面具有局限性。一种新的方法可能是使用一种组织工程结构，这种结构暂时反映了天然韧带组织的机械特性，并作为细胞接种的载体结构。在这项研究中，刺绣支架组成的聚乳酸（PLA）和聚（乳酸-co-ε-己内酯）（P（LA-CL））线的机械测试下，在体外降解的粘弹性行为。两种支架类型（moco：由PLA线制成的单组分支架，bico：由PLA和P（LA-CL）线制成的双组分支架）的松弛行为与天然兔ACL相当。大部分兔ACL细胞在细胞培养32天后存活，并沿纤维沿着生长。moco和bico支架的细胞活力相当。Lapine ACL细胞能够粘附到聚合物表面并沿着线在整个支架上扩散。有细胞和无细胞的降解基质的力学行为没有显着差异。这些结果表明刺绣支架作为ACL组织工程方法的潜力。
A rupture of the anterior cruciate ligament (ACL) is the most common knee ligament injury. Current applied reconstruction methods have limitations in terms of graft availability and mechanical properties. A new approach could be the use of a tissue engineering construct that temporarily reflects the mechanical properties of native ligament tissues and acts as a carrier structure for cell seeding. In this study, embroidered scaffolds composed of polylactic acid (PLA) and poly(lactic-co-epsilon-caprolactone) (P(LA-CL)) threads were tested mechanically for their viscoelastic behavior under in vitro degradation. The relaxation behavior of both scaffold types (moco: mono-component scaffold made of PLA threads, bico: bi-component scaffold made of PLA and P(LA-CL) threads) was comparable to native lapine ACL. Most of the lapine ACL cells survived 32 days of cell culture and grew along the fibers. Cell vitality was comparable for moco and bico scaffolds. Lapine ACL cells were able to adhere to the polymer surfaces and spread along the threads throughout the scaffold. The mechanical behavior of degrading matrices with and without cells showed no significant differences. These results demonstrate the potential of embroidered scaffolds as an ACL tissue engineering approach.