Cuticular collagen genes from the parasitic nematode Ostertagia circumcincta
Cuticular collagen genes from the parasitic nematode Ostertagia circumcincta
复制标题
DOI：
10.1016/0166-6851(96)02682-5
复制
发表时间：
1996-09-01
影响因子：
1.5
通讯作者：
Barry, JD
中科院分区：
文献类型：
--
作者：
Johnstone, IL;Shafi, Y;Barry, JD
线虫表皮是一种多功能结构，其作用包括外骨骼和动物与其环境之间的屏障。它是主要由小的胶原样蛋白组成的细胞外基质。对于这些物种的研究，这些表皮胶原蛋白是由一个多基因家族编码。在自由生活的线虫秀丽隐杆线虫中，这个家族有大约100个成员。我们的数据表明，在寄生线虫Ostertagia circumcincta中存在一个类似大小的基因家族。我们已经确定了一对串联重复的胶原基因从O。colost-1和colost-2是col-12和col-13的直接同源物，col-12和col-13是一对串联重复的C.优美的这些同源物的种间比较表明极端保守的地区。我们的结论是，在C. elegans中产生colost-1和colost-2的复制很可能与O.包皮环切，因此这种特定的基因复制先于两个物种的分歧。这两种线虫的分歧很大，O. circumcincta属于圆虫目（Strongylata），C.从秀丽线虫到杆形动物能够确定直接同源的个别表皮胶原蛋白基因之间的分歧很大的物种提供了一个强大的方法来确定这些小的胶原蛋白的结构重要性的区域。在不同物种中的同源物之间保守的特征，但在一个物种中与多基因家族的其他成员不保守的特征，必须与该特定表皮胶原的特定功能有关。
The nematode cuticle is a multifunctional structure whose roles include exoskeleton and barrier between the animal and its environment. It is an extracellular matrix which consists predominantly of small collagen-like proteins. For those species studied, these cuticular collagens are encoded by a multigene family. In the free living nematode Caenorhabditis elegans, this family has similar to 100 members. Our data indicate a gene family of similar size in the parasitic nematode Ostertagia circumcincta. We have characterised a pair of tandemly duplicated collagen genes from O. circumcincta, colost-1 and colost-2, which we believe to be the direct homologues of col-12 and col-13, a tandemly duplicated pair previously identified in C. elegans. The interspecies comparison of these homologues indicates regions of extreme conservation. We conclude that the gene duplication event that resulted in the creation of col-12 and col-13 in C. elegans is most likely the same duplication that generated colost-1 and colost-2 in O. circumcincta, and thus this particular gene duplication precedes the divergence of the two species. These two nematode species are deeply diverged, O. circumcincta belonging to the order Strongylata and C. elegans to Rhabditata. The ability to identify direct homologues of individual cuticular collagen genes between deeply diverged species provides a powerful method for determining regions of structural importance in these small collagens. Characteristics that are conserved between homologues in divergent species, but not conserved with other members of the multigene family within one species, must relate to the specific function of that particular cuticular collagen.