The isolation and characterization of a calmodulin-encoding gene (CMD1) from the dimorphic fungus Candida albicans.
The isolation and characterization of a calmodulin-encoding gene (CMD1) from the dimorphic fungus Candida albicans.
复制标题
从二态性真菌白色念珠菌中分离和鉴定钙调蛋白编码基因 (CMD1)。
DOI：
10.1016/0378-1119(91)90564-r
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发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
Sypherd,PS
中科院分区：
文献类型：
--
作者：
Saporito,SM;Sypherd,PS
白色念珠菌是人类的二态、机会性病原体，钙和钙调蛋白参与了其形态发生的转变。TheC。以酿酒糖酵母菌CMD1基因为杂交探针，从cDNA和噬菌体λ文库中分离到白色钙调素编码基因CMD1。基因组DNA的Southern-blot杂交分析表明，只有一种类型的钙调蛋白基因存在。cDNA与基因组序列比较，发现一个222 bp的内含子位于Met起始密码子之后。预测的氨基酸序列与酵母CMD的一致性为60%，与丝状真菌和脊椎动物的CMD的一致性为70%。我们利用等高线夹紧均匀电场凝胶电泳将md1基因定位在3号染色体上。md1基因与一个650个核苷酸的转录物杂交，该转录物在酵母和菌丝形式的生物体中都以相同的数量存在。
Candida albicansis a dimorphic, opportunistic pathogen of humans, and calcium and calmodulin have been implicated in its morphogenic transition. TheC. albicanscalmodulin-encoding gene,CMD1, was isolated from cDNA and genomic phage λ libraries using theSaccharomyces cerevisiae CMDgene as a hybridization probe. Southern-blot hybridization analysis of genomic DNA suggests the existence of only one type of calmodulin gene inC. albicans. Comparison of cDNA and genomic sequences identified a 222-bp intron located immediately after the Met start codon. The predicted amino acid sequence was 60% identical with yeast CMD and 70% identical with CMDs of filamentous fungi and vertebrates. We have localized theCMD1 gene to chromosome 3 using the contour-clamped homogeneous electric field gel electrophoresis. TheCMD1 gene hybridized to a single 650-nucleotide transcript which was present in equivalent amounts in both the yeast and hyphal forms of the organism.