A Chromosome-Scale Assembly of the Asian Honeybee Apis cerana Genome
A Chromosome-Scale Assembly of the Asian Honeybee Apis cerana Genome
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DOI：
10.3389/fgene.2020.00279
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发表时间：
2020-03
影响因子：
3.7
通讯作者：
Zi-long Wang;Yong-Qiang Zhu;Qing Yan;W. Yan;Hua-Jun Zheng;Z. Zeng
中科院分区：
文献类型：
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作者：
Zi-long Wang;Yong-Qiang Zhu;Qing Yan;W. Yan;Hua-Jun Zheng;Z. Zeng
中华蜜蜂是人工养殖的主要蜂种之一，广泛分布于亚洲各国。A.几个不同的研究小组使用第二代测序技术对cerana进行了测序。然而，仍然需要获得更完整和准确的基因组序列。在这里，我们提出了一个染色体规模组装的A。使用单分子实时（SMRT）Pacific Biosciences测序和高通量染色质构象捕获（Hi-C）基因组支架，更新后的组装体大小为215.67 Mb，重叠群N50为4.49 Mb，比之前基于Illumina的版本提高了212倍。Hi-C支架导致16个假染色体，占组装基因组序列的97.85%。共预测了10，741个蛋白质编码基因，注释了9，627个基因。此外，与以前的版本相比，确定了314个新基因。改良后的优质A. cerana参考基因组将为中华按蚊的生物学研究提供精确的序列信息。cerana。
Apis cerana is one of the main honeybee species in artificial farming, which is widely distributed in Asian countries. The genome of A. cerana has been sequenced by several different research groups using second generation sequencing technologies. However, it is still necessary to obtain more complete and accurate genome sequences. Here we present a chromosome-scale assembly of the A. cerana genome using single-molecule real-time (SMRT) Pacific Biosciences sequencing and high-throughput chromatin conformation capture (Hi-C) genome scaffolding. The updated assembly is 215.67 Mb in size with a contig N50 of 4.49 Mb, representing an 212-fold improvement over the previous Illumina-based version. Hi-C scaffolding resulted in 16 pseudochromosomes occupying 97.85% of the assembled genome sequences. A total of 10,741 protein-coding genes were predicted and 9,627 genes were annotated. Besides, 314 new genes were identified compared to the previous version. The improved high-quality A. cerana reference genome will provide precise sequence information for biological research of A. cerana.