EXPRESSION OF HEPARIN-BINDING EPIDERMAL GROWTH-FACTOR IN HUMAN HEPATOCELLULAR-CARCINOMA
EXPRESSION OF HEPARIN-BINDING EPIDERMAL GROWTH-FACTOR IN HUMAN HEPATOCELLULAR-CARCINOMA
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DOI：
10.1016/0016-5085(94)90823-0
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发表时间：
1994-12-01
期刊：
影响因子：
29.4
通讯作者：
MATSUZAWA, Y
中科院分区：
文献类型：
--
作者：
INUI, Y;HIGASHIYAMA, S;MATSUZAWA, Y
背景/目的：生长因子参与癌症的发生和发展。本研究的目的是评估肝素结合表皮生长因子样生长因子（HB-EGF），这是EGF家族的成员，在肝细胞的肿瘤转化的可能作用。研究方法：应用北方杂交和免疫组化方法，检测了17例人肝细胞癌（HCC）组织中HB-EGF基因表达和蛋白表达。结果：HCC组织中HB-EGF mRNA的表达量较癌旁肝组织明显增加。在非癌肝组织中，HB-EGF在肝细胞中呈弱阳性。在所有17例HCC患者的HCC细胞中鉴定出免疫反应性HB-EGF产生细胞，表明HB-EGF在HCC细胞本身中产生。10例肝转移腺癌患者的肝组织中无HB-EGF阳性。与HB-EGF结合的EGF受体也在HCC细胞上表达。结论：据推测，在细胞上的免疫反应性HB-EGF的表达增强表明HB-EGF在人类HCC的发展或进展中的自分泌和/或分泌方式的可能作用。
Background/Aims: Growth factors are involved in the development and progression of cancer. The purpose of this study was to evaluate the possible role of heparin-binding epidermal growth factor-like growth factor (HB-EGF), which is a member of the EGF family, in the neoplastic transformation of hepatocytes. Methods: Gene expression and protein production of HB-EGF were investigated in samples of human hepatocellular carcinoma (HCC) from 17 patients using Northern hybridization and immunohistochemical methods. Results: The amount of HB-EGF messenger RNA was increased in the patients' HCC specimens compared with the surrounding liver tissues. In noncancerous hepatic tissues, HB-EGF was faintly positive in hepatocytes. Immunoreactive HB-EGF-producing cells were identified in HCC cells of all 17 patients with HCC, indicating that HB-EGF was produced in HCC cells themselves. However, none of the specimens from 10 patients with metastatic adenocarcinoma in the liver was positive for HB-EGF. The EGF receptor, which binds to HB-EGF, was also expressed on HCC cells. Conclusions: It is hypothesized that the enhanced expression of immunoreactive HB-EGF on the cell suggests a possible role of HB-EGF in the development or progression of human HCC in an autocrine and/or a juxtacrine manners.