Induction of cyclooxygenase-2 mRNA and protein following transient focal ischemia in the rat brain
Induction of cyclooxygenase-2 mRNA and protein following transient focal ischemia in the rat brain
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DOI：
10.1016/0304-3940(95)12108-g
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发表时间：
1995-11-24
影响因子：
2.5
通讯作者：
Ferrer, I
中科院分区：
文献类型：
--
作者：
Planas, AM;Soriano, MA;Ferrer, I
应用原位杂交技术研究了大鼠大脑中动脉（MCA）缺血1h后30 min和4 h脑内环氧合酶-2（考克斯-2）mRNA和诱导型热休克蛋白-70（hsp-70）mRNA的表达。另外，在再灌注8小时后研究了考克斯-2的免疫反应性。Hsp-70和考克斯-2 mRNA在大脑中动脉闭塞的同侧被发现的诱导。Hsp-70 mRNA诱导顶叶皮质和纹状体内的闭塞MCA的领土。考克斯-2 mRNA的诱导在大脑同侧MCA闭塞的皮质层II中特别可见。在再灌注30分钟，显示考克斯-2 mRNA的诱导区域包括位于hsp-70 mRNA诱导区域周围的扣带回和额叶皮质，以及梨状皮质。再灌注4 h，顶叶皮质内可见考克斯-2 mRNA的诱导表达。再灌注8h，考克斯-2主要表达于同侧皮质。这些结果表明，短暂局灶性脑缺血诱导考克斯-2 mRNA和蛋白在脑缺血再灌注过程中的表达，这可能导致局部花生四烯酸代谢增加
Expression of cyclooxygenase-2 (cox-2) mRNA and inducible heat-shock protein-70 (hsp-70) mRNA was studied with in situ hybridization techniques at 30 min and 4 h following 1 h transient middle cerebral artery (MCA) occlusion in the rat brain. In addition, immunoreactivity for cox-2 was studied after 8 h of reperfusion. Induction of hsp-70 and cox-2 mRNA was found in the brain side ipsilateral to MCA occlusion. Hsp-70 mRNA was induced in the parietal cortex and striatum within the territory of the occluded MCA. Induction of cox-2 mRNA was particularly seen in cortical layer II in the brain side ipsilateral to MCA occlusion. At 30 min of reperfusion, areas showing cox-2 mRNA induction included the cingulate and frontal cortices located perifocally to the areas showing hsp-70 mRNA induction, and the piriform cortex. At 4 h of reperfusion, induction of cox-2 mRNA was seen within the parietal cortex. At 8 h of reperfusion, immunoreactivity for cox-2 was mainly seen in the ipsilateral cortex. These results demonstrate that transient focal ischemia induces the expression of cox-2 mRNA and protein in discrete areas of the rat brain during reperfusion, which might lead to local increases of arachidonic acid metabolism