EFFECTS OF CHLOROQUINE AND CYTOCHALASIN-B ON THE INFECTION OF CELLS BY SINDBIS VIRUS AND VESICULAR STOMATITIS-VIRUS
EFFECTS OF CHLOROQUINE AND CYTOCHALASIN-B ON THE INFECTION OF CELLS BY SINDBIS VIRUS AND VESICULAR STOMATITIS-VIRUS
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DOI：
10.1128/jvi.37.3.1060-1065.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
BROWN, DT
中科院分区：
文献类型：
--
作者：
COOMBS, K;MANN, E;BROWN, DT
用电镜观察了细胞松弛素B和氯喹对辛德毕斯病毒颗粒和聚苯乙烯球内吞过程的影响。测定了这些试剂对辛德毕斯病毒和水泡性口炎病毒感染BHK-21（幼仓鼠肾）细胞的过程（附着、穿透和脱壳）的影响。细胞松弛素B完全阻断了BHK细胞对辛德毕斯病毒颗粒或乳胶球的摄取，但对辛德毕斯病毒或水疱性口炎病毒在BHK细胞中感染或复制的能力没有影响。氯喹不抑制乳胶球或病毒颗粒的摄入，但大大降低了病毒的产量。内吞作用可能不是辛德毕斯病毒或水泡性口炎病毒感染培养细胞所必需的。
The effects of cytochalasin B and chloroquine on the process of endocytosis of Sindbis virus particles and polystyrene spheres were determined by EM. The effects of these agents on the process of infection (attachment, penetration and uncoating) of BHK-21 (baby hamster kidney) cells by Sindbis virus and vesicular stomatitis virus were determined. Cytochalasin B completely blocked ingestion of Sindbis virus particles or latex spheres by BHK cells but had no effect on the ability of Sindbis virus or vesicular stomatitis virus to infect or replicate in BHK cells. Chloroquine did not inhibit the ingestion of latex spheres or virus particles but greatly reduced the yields of virus produced. Endocytosis may not be essential for the infection of cultured cells by Sindbis virus or vesicular stomatitis virus.