DNA Microarray Analysis of Labial Salivary Glands in IgG4-Related Disease
DNA Microarray Analysis of Labial Salivary Glands in IgG4-Related Disease
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DOI：
10.1002/art.38748
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发表时间：
2014-10-01
影响因子：
13.3
通讯作者：
Sumida, Takayuki
中科院分区：
文献类型：
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作者：
Tsuboi, Hiroto;Nakai, Yuji;Sumida, Takayuki
目的比较igg4相关疾病患者唇唾液腺（LSGs）与干燥综合征（SS）患者唇唾液腺（LSGs）的基因表达。方法采用DNA芯片技术对5例igg4相关疾病患者、5例SS患者和3例健康对照的LSG样本进行基因表达分析。鉴定出igg4相关疾病和SS中差异表达的基因，并利用基因本体（gene Ontology， GO）注释对这些差异表达基因进行基因注释富集分析。采用定量聚合酶链反应（PCR）对9例igg4相关疾病患者、10例SS患者和4例对照组的LSG样本进行验证。结果igg4相关疾病患者、SS患者和健康对照组的基因表达谱在层次聚类和主成分分析中存在较大差异。与SS相比，在igg4相关疾病中，共有1771个探针组（对应1321个基因）被鉴定为上调，1785个探针组（对应1320个基因）被鉴定为下调(错误发现率为
ObjectiveTo compare gene expression in labial salivary glands (LSGs) from patients with IgG4-related disease with that in LSGs from patients with Sjogren's syndrome (SS).MethodsGene expression was analyzed by DNA microarray in LSG samples from 5 patients with IgG4-related disease, 5 SS patients, and 3 healthy controls. Genes differentially expressed in IgG4-related disease and SS were identified, and gene annotation enrichment analysis of these differentially expressed genes was performed using Gene Ontology (GO) annotation. Validation of the results was performed by quantitative polymerase chain reaction (PCR) using LSG samples from 9 patients with IgG4-related disease, 10 SS patients, and 4 controls.ResultsGene expression patterns in patients with IgG4-related disease, SS patients, and healthy controls were quite different from each other in hierarchical clustering as well as in principal components analysis. In IgG4-related disease compared with SS, a total of 1,771 probe sets (corresponding to 1,321 genes) were identified as up-regulated, and 1,785 probe sets (corresponding to 1,320 genes) were identified as down-regulated (false discovery rate of