Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips
Acoustic micro-vortexing of fluids, particles and cells in disposable microfluidic chips
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DOI：
10.1007/s10544-016-0097-4
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发表时间：
2016-08-01
影响因子：
2.8
通讯作者：
Wiklund, Martin
中科院分区：
文献类型：
--
作者：
Iranmanesh, Ida;Ohlin, Mathias;Wiklund, Martin
我们展示了用于具有小体积（20μl）反应室的一次性聚合物微流控芯片中的微涡旋的声学平台。所描述的方法被证明适用于各种标准涡旋功能，包括混合流体、重新悬浮由放置在芯片上的磁铁收集的磁珠颗粒，以及裂解细胞以提取 DNA。该设备基于改进的朗之万型超声波换能器，带有指数喇叭，可有效耦合到微流控芯片中，微流控芯片由低成本固定频率电子驱动板驱动。通过数值建模对换能器进行优化，并通过驱动换能器不同的时间来实现不同的涡旋功能；从流体混合的几分之一秒到细胞裂解和 DNA 提取的半分钟。借助 PC 风扇、珀耳帖元件和充当芯片支架的铝制散热器，该平台可以在低于生理温度的 1 分钟内运行。作为样品制备应用的原理证明，我们演示了 25 秒内的片上细胞裂解和 DNA 提取。该方法对于各种生物测定中的自动化和芯片集成样品制备程序很有意义。
We demonstrate an acoustic platform for microvortexing in disposable polymer microfluidic chips with small-volume (20 mu l) reaction chambers. The described method is demonstrated for a variety of standard vortexing functions, including mixing of fluids, re-suspension of a pellet of magnetic beads collected by a magnet placed on the chip, and lysis of cells for DNA extraction. The device is based on a modified Langevin-type ultrasonic transducer with an exponential horn for efficient coupling into the microfluidic chip, which is actuated by a low-cost fixed-frequency electronic driver board. The transducer is optimized by numerical modelling, and different demonstrated vortexing functions are realized by actuating the transducer for varying times; from fractions of a second for fluid mixing, to half a minute for cell lysis and DNA extraction. The platform can be operated during 1 min below physiological temperatures with the help of a PC fan, a Peltier element and an aluminum heat sink acting as the chip holder. As a proof of principle for sample preparation applications, we demonstrate on-chip cell lysis and DNA extraction within 25 s. The method is of interest for automating and chip-integrating sample preparation procedures in various biological assays.