Investigating the Role of Specific Tear Film Lipids Connected to Dry Eye Syndrome: A Study on O-Acyl-ω-hydroxy Fatty Acids and Diesters
Investigating the Role of Specific Tear Film Lipids Connected to Dry Eye Syndrome: A Study on O-Acyl-ω-hydroxy Fatty Acids and Diesters
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DOI：
10.1021/acs.langmuir.8b04182
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发表时间：
2019-03-05
期刊：
影响因子：
3.9
通讯作者：
Paananen, Riku O.
中科院分区：
文献类型：
--
作者：
Bland, Helena C.;Moilanen, Jukka A.;Paananen, Riku O.
干眼综合征（DES）是一种常见的泪膜内稳态受损的疾病。DES的主要原因之一被认为是泪膜的最外层（泪膜脂质层（TFLL））的组成的改变，导致水从泪膜的蒸发增加和随后的眼表面干燥。最近的研究表明，特定的TFLL脂质，即O-芳基-ω-羟基脂肪酸（OAHFA）和二酯（DiE），可能在DES的发展中发挥作用。然而，他们的具体连接DES仍然在很大程度上是未知的，直到现在，因为缺乏信息，他们的生物物理特性和TFLL中的作用。在这里，我们已经解决了这个问题，通过研究的生物物理特性和蒸发阻力的图书馆含有10个合成类似物的TFLL OAHFA和二酯。我们的结果显示了链长和极性基团的变化如何影响这些脂质在泪膜表面的相行为。此外，结果表明，表现出液体膨胀到固体相变的OAHFA形成具有高蒸发阻力的膜，而发现DiE没有蒸发阻力。总之，我们的研究结果揭示了OAHFA和DiE在TFLL中的作用及其与DES的联系，表明OAHFA可能是维持TFLL蒸发阻力的关键脂质类。
Dry eye syndrome (DES) is a prevalent disease in which the tear film homeostasis is compromised. One of the main causes of DES is thought to be an alteration in the composition of the outermost layer of the tear film, the tear film lipid layer (TFLL), resulting in an increased evaporation of water from the tear film and subsequent drying of the ocular surface. Recent studies have suggested that the specific TFLL lipids, namely, O-aryl-omega-hydroxy fatty acids (OAHFAs) and diesters (DiEs), may play a role in the development of DES. However, their specific connection to DES has remained largely unknown until now because of the lack of information on their biophysical properties and their role in the TFLL. Herein, we have addressed this issue by studying the biophysical properties and evaporation resistance of a library containing 10 synthetic analogues of TFLL OAHFAs and DiEs. Our results show how the variations of chain length and polar groups affect the phase behavior of these lipids at the tear film surface. In addition, the results revealed that the OAHFAs exhibiting a liquid-expanded to solid phase transition formed films with high evaporation resistance, whereas the DiEs were found to have no evaporation resistance. Altogether, our results shed new light on the role of the OAHFAs and DiEs in the TFLL and their connection to DES, suggesting that OAHFAs are likely a key lipid class in maintaining the TFLL evaporation resistance.